Influence of soil physicochemical properties on microbial diversity in different production areas of Chinese Cordyceps in Qinghai province
[Objective]The present paper aimed to explore the relationship between soil physical and chemical factors and microorganisms in Chinese Cordyceps producing areas of Qinghai province,in order to provide reference for the further improvement of artificial Chinese Cordyceps culture matrix.[Method]Hualong Hui Nationality Autonomous county(HL)in Haidong city,Henan Mongol Autonomous county(HN)in Huangnan Tibetan Autonomous Prefecture,Xinghai county(XH)in Hainan Tibetan Autonomous Prefecture,Dari county(DR)in Golog Tibetan Autonomous Prefecture,Maqin county(MQ)in Golog Tibetan Autonomous Prefecture,Zaduo county(ZD)in Yushu Tibetan Autonomous Prefecture,and Guoqing Ranch(GQ)in Yushu Tibetan Autonomous Prefecture were selected as the experimental plots.Potassi-um dichromate-external heating method,kjeldahl nitrogen determination method,alkaline fusion method,acid dissolution method,o-phenanth-roline colourimetric method,flame atomic absorption spectrophotometry,flame photometric method and potassium periodate colourimetric method were used for the determination of the physical and chemical properties of the soil.Microbial content and species were determined u-sing high-throughput sequencing technology.[Result]Most of the soil physical and chemical factors were not significantly different in different producing areas.The composition of microorganisms in each producing area was similar,and the diversity and richness of fungal dominant communities were different among different producing areas.In the bacterial community,Proteobacteria,Actinobacteria and Acidobacteria were the dominant bacterial phyla,and Subgroup_6,Pseudomonas and RB41 were the dominant bacterial genera;In the fungal community,Ba-sidiomycota,Ascomycota and Mortierellomycota were the dominant fungal phyla,and Hygrocybe,Sebacina and Mortierella were the dominant fungal genera.Redundancy analysis was performed on soil physical and chemical properties and microbial communities in the production are-a.Results showed that Actinobacteria highly significant positive correlation(P＜0.01)with soil pH and elemental iron(Fe),while RB41,Nitrospira and Subgroup_6 significant negative correlation(P＜0.05)with elemental total potassium(TK),total phosphorus(TP),organic matter(OM),manganese(Mn)and sodium(Na);The fungus Mortierellomycota was positively correlated with soil total phosphorus(TP),Ascomycota was negatively correlated with soil total potassium(TK),total nitrogen(TN)and organic matter(OM),and soil pH was posi-tively correlated with the dominant fungal community at most genus levels.[Conclusion]The study further confirmed that there is significant correlation between soil physical and chemical factors and microorganisms.Among them,soil pH,organic matter(OM),sodium(Na)and i-ron(Fe)are significantly correlated with most microbial communities in Chinese Cordyceps producing areas(P＜0.05),which may be the key driving factors affecting microbial community structure.
Chinese CordycepsOrganic matterTotal nitrogenTotal phosphorusSoil microorganisms
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