Characteristics of rhizosphere soil microbial communities in Gentiana rigescens Franch.based on high-throughput sequencing
[Objective]The paper aimed to provide scientific and effective theoretical basis for understanding the rhizosphere soil microbial community structure,making full use of microbial resources and cultivating Gentiana rigescens Franch.with good quality.[Method]The mi-crobial diversity and community structure of rhizosphere soil of G.rigescens Franch.from different producing areas were compared and ana-lyzed by high-throughput sequencing technology.The correlation between microbial community abundance and soil nutrient content in rhizo-sphere soil was analyzed in combination with soil physicochemical properties.[Result]There were differences in the diversity and richness of rhizosphere soil microorganisms in different areas,among which the Chao1,Ace and Shannon indices of sample BS2 were the largest.The a-nalysis of community composition showed that the bacteria belonged to 136 phylums,86 classes,188 orders,409 families and 1793 genera.The dominant bacteria of D12 and LC were Proteobacteria,and their relative abundance was 64.74％and 56.00％,respectively.Acidobacte-ria were the dominant bacteria in samples DL1,BS1,BS2 and GZ,and their relative abundance was 42.02％,24.40％,41.44％and 71.30％,respectively.Fungi of rhizosphere soil belonged to 5 phylums,28 classes,63 orders,124 families and 181 genera.Basidiomycota(42.65％)was the dominant fungus in sample BS2,and Ascomycota(48.50％-88.92％)was the dominant fungus in the other five sam-ples.Correlation analysis of rhizosphere microorganisms and soil physicochemical properties showed that soil pH was significantly positively correlated with the relative abundance of Streptomyces and negatively correlated with Candidatus-Sulfotelmatter.The organic matter content was significantly positively correlated with the relative abundance of both Edaphobacter and Granulicella.Soil available nutrients such as hy-drolyzed nitrogen,available phosphorus and available potassium were significantly or extremely significantly positively correlated with the rela-tive abundance of Rhodanobacter.[Conclusion]The origin factors have a certain effect on the microbial diversity and community structure of rhizosphere soil of G.rigescens.Comprehensively,sample BS2 has the highest richness and diversity of rhizosphere soil microorganisms.Pro-teobacteria,Acidobacteria,Actinobacteria and Chloroflexi are the dominant bacteria in all samples,while Ascomycota and Basidiomycota are the dominant fungal communities.
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