Effect of biochar on soil metabolic markers in cotton fields under saline irrigation
[Objective]The present paper aimed to further clarify the metabolic mechanism of applying biochar to soil under long-term saline irrigation,and to explore and analyze the differences in the composition of metabolic markers in biochar applied soil.[Method]The experi-ment was set with two irrigation water salinities,fresh water(FW)and saline water(SW),and biochar was applied at each water salinity,re-presented by FWBC and SWBC.The metabolomics method of gas chromatography-time-of-flight mass spectrometry(GC-TOF-MS)was used to study the composition of metabolites in cotton field soil.[Result]Under freshwater irrigation conditions,the application of biochar signifi-cantly increased the soil moisture content,electrical conductivity,pH,total carbon,available potassium,and available phosphorus by 30.4％,14.3％,1.4％,22.8％,39.9％and 18.7％compared to the treatment without biochar;Under saline irrigation conditions,the application of biochar significantly increased the content of total carbon,total nitrogen,available potassium,and available phosphorus in the soil by 9.8％,4.8％,33.7％and 48.7％compared to the treatment without biochar,but the soil conductivity significantly decreased by 14.5％.Under freshwater irrigation,biochar was applied and a total of 5 metabolites with significant differences(P＜0.05,the same as below)were select-ed,all of which showed downregulation;Under saline water irrigation,22 metabolites with significant differences were screened by applying biochar,all of which showed upregulation.The classification of soil metabolites mainly included lipid and lipid molecules,organic acids and their derivatives,organic oxygen compounds,benzene ring compounds,organic heterocyclic compounds,phenylpropanoids,and polyketone me-tabolites.Among them,furoic acid and niacin screened under saline irrigation conditions belong to the organic heterocyclic compounds,lactu-lose and 2-aminophenol belong to the organic oxygen compounds,and 3-hydroxy-2-aminobenzoic acid belongs to the benzene ring compounds.[Conclusion]The application of biochar can improve soil physical and chemical properties,increase the types and abundance of metabolites in saline irrigated soil,effectively alleviate the harm caused by long-term saline irrigation,and provide certain ideas for the rational develop-ment and utilization of saline water resources in arid areas.
Salt water irrigationBiocharSoilMetabolomicsMetabolites
万方数据
维普
