Identification and expression analysis of ZF-HD transcription factors of hemp
[Objective]The ZF-HD(Zinc finger-homeodomain)transcriptional factors of hemp were identified and their expression patterns were analyzed to provide theoretical reference for ZF-HD function research and improvement of hemp genetic traits.[Method]The gene struc-tures,protein physicochemical properties,protein phylogenetic relationships and promoter cis-elements of five ZF-HD transcription factors in hemp were analyzed by bioinformatics,and the gene expression levels were analyzed by real-time fluorescent quantitative PCR(qRT-PCR).[Result]Five CsZF-HD genes were distributed on two chromosomes of hemp genome.Their coding sequence lengths were 783-1185 bp,encoding 260-394 amino acids residues.The molecular weights of five CsZF-HD proteins were 27.95-43.33 ku,and the theoretical isoe-lectric points were 7.46-8.87.Five CsZF-HD proteins all contained a conserved ZF-HD_dimer domain and were unstable hydrophilic pro-teins localized in the nucleus.The ZF-HD protein family was divided into three subfamilies(A,B and C).CsZF-HD1 and CsZF-HD2 be-long to the A subgroup,CsZF-HD3 belongs to the C subgroup,and CsZF-HD4 and CsZF-HD5 belong to the B subgroup.The expression lev-els of CsZF-HD1 and CsZF-HD2 in stems and leaves were not significantly different,but the expression levels in roots were the lowest.The expression level of CsZF-HD3 was the highest in stems and the lowest in leaves.The expression of CsZF-HD4 was the highest in leaves and the lowest in roots.The expression level of CsZF-HD5 was the highest in roots and the lowest in stems.All the five CsZF-HD genes were re-sponsive to salt and drought stresses,and the expression levels of CsZF-HD1 and CsZF-HD3 were most significantly increased under salt and drought stresses.The promoters of five CsZF-HD genes contained different types and quantities of stress-related cis-elements.[Conclusion]ZF-HD transcription factors may play a role in the transcriptional regulation of hemp in response to salt and drought stresses.
万方数据
维普
