Effect of synergistic action of pH and magnesium ions on chlorophyll biosynthesis in tea trees
[Objective]The synthesis pattern of tea tree chlorophyll by synergistic effect of soil pH and magnesium ions was explored to pro-vide theoretical basis for the cultivation of tea tree and breeding of new variety.[Method]Taking Fuding Dabaicha as the study object,the plant tissue enzyme activity kits were used to determine the synergistic effects of the soil with different pH[pH 4(P1)and pH 5(P2)]and different magnesium ion concentrations[0 mg/L(To,CK),50 mg/L(T1),100 mg/L(T2)and 150 mg/L(T3)]on the key enzymes of chlorophyll synthesis in tea tree,including magnesium chelatase,uroporphyrinogen Ⅲ synthase and 5-amino ketoglutarate dehydratase.The high-throughput technology was used for transcriptomic sequencing of the leaf slices tissue of Fuding Dabaicha with different treatments,and then the synergistic effect of soil pH and magnesium ion on the synthesis of chlorophyll in tea tree were studied.[Result]The soil pH and magnesium ions had different synergistic effects on the key enzymes of chlorophyll synthesis in tea tree(magnesium chelatase,uroporphyrino-gen Ⅲ synthase and 5-amino ketoglutarate dehydratase).The activities of magnesium chelatase,uroporphyrinogen Ⅲ synthase and 5-amino ketoglutarate dehydratase treated with P2T1 were the highest with 492.41,336.28 and 132.24 U/L,respectively.The analysis of GO and KEGG functional enrichment showed that the key regulatory genes in the development of Fuding Dabaicha leaves were mainly involved in the processes of carbohydrate metabolism and polysaccharide metabolism.The differentially expressed genes were mainly enriched in starch and sucrose metabolism,stilbenes and other pathways.The analysis of KEGG metabolic pathway of photosynthesis of carbon fixation pathway showed that P2T1 treatment significantly improved the carbon fixation pathway of tea tree,and had a promotional effect on both photosynthesis and sugar accumulation.[Conclusion]P2T1 treatment(the synergistic effects of pH 5 and 50 mg/L magnesium ion)of tea tree has the best effect on chlorophyll synthesis,which is beneficial to the synthesis and accumulation of materials,and is advantageous to the quality and yield of tea tree.
Fuding DabaichapHMagnesium ion concentrationChlorophyll synthesisTranscriptome
万方数据
维普
