Fungal virus diversity in rice sheath blight pathogen AG-Bb
[Objective]The purpose of the experiment was to explore the virus species of Rhizoctonia solani AG-Bb fusion subgroup in Yun-nan,screen for fungal viruses that could effectively reduce the pathogenicity of R.solani,and provided biocontrol virus resources for the pre-vention and control of R.solani.[Method]Next-generation sequencing(NGS)was used to detect the virus species in R.solani AG-Bb fusion subgroup strains 8-Q-36,2-R-11 and 2-Q-13,and identif viruses that could reduce host pathogenicity by the ribavirin high-temperature tip detoxification method.[Result]The results of sequence analysis of strains 8-Q-36,2-R-11 and 2-Q-13 were compared to six mycoviridae,Partitiviridae,Endornaviridae,Fusariviridae,Mitoviridae,Rhabdoviridae,Totiviridae,and some unclassified viruses.Compared with the o-riginal strain 8-Q-36,the colony morphology of the detoxified strain 8-Q-36-1 was more fluffy,the sclerotia exhibited obvious pigmentation,and the pathogenicity was significantly enhanced.Double-stranded RNA(dsRNA)extraction and detection showed that the largest≥10 kb dsRNA deletion in the detoxified strain 8-Q-36-1,which led to the determination that the dsRNA could reduce host pathogenicity.After NC-BI comparison,it was found that part of the sequence of the dsRNA was RdRp sequence,and it had homology with the RdRp sequence of the endosomic viridae family,and the identity with the sequence of RsEV6,the virus with the highest similarity,was 56.02％,so the isolate of strain 8-Q-36 might be a new virus,tentatively named RsEV11.[Conclusion]The 8-Q-36,2-R-11 and 2-Q-13 strains of R.solani AG-Bb fusion subgroup have rich viral diversity,among which biocontrol virus isolates capable of reducing the pathogenicity of R.solani are present in strain 8-Q-36.In the study,fungal virus with biocontrol value are indentified for the first time in the genus Ceratobasidium spp.
万方数据
维普
