Effects of different substrate ratios on substrate environment in cucumber rhizosphere and bacterial community structure
[Objective]The study aimed to explore the changes of rhizosphere matrix environment and bacterial community structure in differ-ent proportions of grape waste branches compound matrix during cucumber cultivation,and to provide theoretical and technical support for the matrix utilization of grape abandoned branches.[Method]Cucumber was used as the research object to determine the substrate nutrients and enzyme activity characteristics of five treatments[CK:commercial substrate,T1:v(grape branch fermentation)∶v(peat)∶v(perlite)=2∶5∶3,T2:v(grape branch fermentation)∶v(peat)∶v(perlite)=3∶4∶3,T3:v(grape branch fermentation)∶v(peat)∶v(perlite)=4∶3∶3,T4:v(grape branch fermentation)∶v(peat)∶v(perlite)=5∶2∶3],and Illumina MiSeq high-throughput sequencing technology was used to explore the changes of bacterial community structure.[Result]Compared with CK,the nutrients of grape waste branches compound matrix were significantly different.The contents of available nitrogen and available phosphorus in compound matrix increased significantly,and the activities of phosphatase,urease,catalase and cellulase were significantly improved.The results of bacterial community composition showed that the diversity and richness index of cucumber rhizosphere bacterial community cultivated with compound substrate were significantly in-creased,and the increase of T4 treatment was the most significant.The bacterial community species composition of different treatments was the same,but the bacterial community structure of the compound matrix treatment was significantly different from that of CK.The dominant bacteria of each treatment were Actinobacteria,Proteobacteria,Chloroflexi and Acidobacteria,accounting for 75.27％-81.33％of the total abundance.The results of correlation analysis showed that available nitrogen,available phosphorus and available potassium were the main factors affecting bacterial diversity.[Conclusion]Different substrate ratios significantly affect substrate nutrients,bacterial diversity and pop-ulation structure.Among them,the substrate nutrient characteristics,enzyme activity and bacterial diversity and richness of T3 and T4 treat-ments are better,which can be used as the optimal substrate ratio for cucumber cultivation.
Abandoned grape branchesCompound matrixMatrix nutrientsEnzyme activityBacterial community diversity
万方数据
维普
