[Objective]The present study aimed to screen candidate genes related to the metabolism of lip pigment in goats with different col-ors screened by RNA-Seq technology,and the molecular mechanism of the color difference of goat lip mucosa was initially revealed,which provided theoretical basis for the study of animal mucosal pigment metabolism.[Method]The lip mucosa tissues of Youzhou Dark-skin goats(black)and Banjiao goats(pink)with different facial mucosal color were selected as the study objects.The transcriptome sequencing was performed by Illumina Hiseq 2000 sequencing platform.After quality control and assembly,the differentially expressed genes were screened using|log2(Fold Change)|＞1 and q value＜0.05 as the criteria.Functional annotation and enrichment analysis of differentially expressed genes were performed.[Result]A total of 249 differentially expressed genes were screened by RNA-Seq technology.Compared with the pink mucosal tissues,218 up-regulated genes and 31 down-regulated genes were found in the black mucosal tissues.A total of 23 differentially ex-pressed genes were predicted to be classified into 9 TFs families,of which 14 genes were classified into transcription factor families contai-ning zinc finger domains.GO functional enrichment analysis showed that differentially expressed genes were mainly involved in various ion transport-related biological processes and molecular functions.The enrichment results of KEGG metabolic pathways showed that the differen-tially expressed genes were significantly enriched in oxytocin,calcium,cGMP-PKG signaling pathways,and other 7 signaling pathways relat-ed to muscle tissue function,include hypertrophic cardiomyopathy and cardiac muscle contraction,among which calcium and cGMP-PKG signaling pathways were closely related to pigment metabolism.In addition,12 genes including ASIP were enriched into the core metabolic pathways of pigment such as MAPK signaling pathway,cAMP signaling pathway,and Melanogenesis signaling pathway.Four differentially expressed genes,including CALML6,OXTR,ATP1A2 and ATP1B4 were simultaneously enriched into multiple pathways related to pigment metabolism.qRT-PCR results were consistent with sequencing results,indicating that the sequencing results were reliable.[Conclusion]ASIP gene is preliminarily identified as a candidate gene for pigmentation metabolism of goat lip.Four genes,including CALML6,OXTR,ATP1A2 and ATP1B4,are involved in pigmentation process of goat lip mucosa,which provide theoretical basis for studying the molecular mechanism of pigmentation metabolism of goat mucosa.It also provides reference for exploring the molecular mechanism of abnormal colora-tion of human mucous membranes.
维普
万方数据
