Rhizosphere soil fungal community structure of different varieties of tobacco with black shank disease
[Objective]The paper aimed to explore the interaction between rhizosphere soil fungi and different tobacco varieties,and reveal significant differences in species among different varieties of diseased rhizosphere soil fungi,as to provide reference for the study of resistance mechanism of tobacco varieties and biological pest control.[Method]Illumina MiSeq high-throughput sequencing technology was used to an-alyze the composition and diversity of fungal community in rhizosphere soil of diseased plants of 8 tobacco varieties in Yunnan province.[Re-sult]The OTU abundance of fungi in diseased rhizosphere soil of different varieties ranged from 822 to 1126.There were significant differ-ences in the diversity and richness index of rhizosphere soil fungi among different varieties,and varieties K326,Yun 105,Yun 87 and Yun 99 were significantly higher than other varieties.At phylum level,the dominant fungi in the diseased rhizosphere soil of each variety were Ascomycota,Mortierellomycota and Basidiomycota.At the genus level,Microidium,Fusarium,Humicola,Mortierella and Alternaria were the dominant genera in each treatment group.The significant difference analysis of LEFSe showed that Humicola,Candida,Plectospha-erella,Alternaria,Papulaspora,Fusarium,Boeremia,Mortierella,Gibberella were the important significantly different species in the dis-eased rhizosphere soil of different varieties.[Conclusion]There are significant differences in fungal community structure and diversity in rhi-zosphere soil of different varieties,and the distribution of significantly different species is related to the resistance of varieties.Among the va-rieties with high resistance to black shank disease(Yun 97,K326,Yun 100 and Yun 87),beneficial bacteria are mainly present,while in susceptible and moderately resistant varieties(Hongda,Yun 105,Yun 116 and Yun 99),harmful bacterial groups such as pathogenic bacteria are clustered.
Tobacco black shankVarietiesRhizosphere soilFungal community
NETL
NSTL
万方数据
