Physiological effects on pepper seedlings and fruits induced by GABA and BABA to anthrax disease
[Objective]The study aimed to investigate the effect of γ-amino-butyric acid(GABA)and β-amino-butyric acid(BABA)on pepper seedlings and pepper fruits.[Method]'Hongguan No.3'and'Chuanteng No.10'pepper cultivars were utilized as test materials.Pepper seedlings were cultured in 1/2 MS solid medium,and pre-treated with 50 mmol/L GABA and 50 mmol/L BABA(GABA and BABA were not added as non-pre-treatment control)to analyze physiological changes after inoculation with Colletotrichum socvillei(inoculated with H2O as non-inoculation control).Meanwhile,red fruit of peppers were pretreated with 50 mmol/L GABA and 50 mmol/L BABA to analyze the physiological changes after C.socvillei.[Result]After inoculation C.socvillei,the leaves of both'Hongguan No.3'and'Chuanteng No.10'pepper seedlings exhibited a decreasing level in chlorophyll a and b as days past inoculations.This decline was significant when compared to the non-inoculated control on 8-day(H2O).(P＜0.05,the same as below).The root growth rate of these two pepper cultivars also showed downtrends with days past inoculation;Both GABA and BABA pre-treatment groups showed a reduced decline in chlorophyll content and root length compared to the non-pre-treatment control group after inoculation with C.socvillei,separately.The chlorophyll con-tent of GABA pre-treatment group remained consistently higher than that of the other treatment groups,with a significant difference observed at 2-day past inoculation.At 8 days past inoculation,the POD activities of GABA and BABA pre-treatment groups were significantly lower than those of the non-pre-treatment control group separately.Moreover,the MDA contents of GABA and BABA pre-treatment groups were significantly lower than that of the non-pre-treatment control group.Furthermore,there were minimal significant differences within each GA-BA and BABA pre-treatment group concerning antioxidant enzymes or MDA levels when comparing from 0-day to 8-day past inoculation.However,the antioxidant enzymes activities of GABA and BABA pre-treatment groups were consistently higher than those of the non-pre-treatment control group 0 days,and MDA contents of both groups remained comparable to the non-pre-treatment control group 0 days;At 8 days past-inoculation,the POD and SOD activities of GABA and BABA pre-treatment groups with red pepper fruits were significantly lower than those of the non-pre-treatment control group,and the MDA contents of GABA and BABA pre-treatment groups were significantly lower than that of the non-pre-treatment control group separately.Additionally,the lesion diameter of GABA and BABA pre-treatment groups were significantly reduced by 1.34 and 0.68 times,respectively,compared to the non-pre-treatment control group.In addition,the trends in the variation of antioxidant enzymes activities and MDA contents within red pepper fruits pre-treatment groups compared 0-day to 8-day past inoc-ulation were consistent with the results observed in pepper seedlings.[Conclusion]The results show that GABA and BABA increase the leaf chlorophyll content and root growth rate of pepper seedlings after inoculation with C.socvillei.Meanwhile,GABA and BABA enhance dis-ease resistance by activating the antioxidant enzyme system in seedlings and pepper fruits and reducing the accumulation of MDA.This en-hancement improves the resistance to anthracnose in both pepper seedlings and fruits.
NETL
NSTL
万方数据
