热带雨林树种陆均松和青梅根际真菌群落多样性分析
Diversity analysis of rhizosphere fungal community of Dacrydium pectinatum and Vatica mangachapoi in tropical rain forest
王欣 1兰国玉 2舒常庆 3魏亚情 2许心诺2
作者信息
- 1. 华中农业大学园艺林学学院,武汉 430070;热带农业科学院橡胶研究所,海口 571101
- 2. 热带农业科学院橡胶研究所,海口 571101;农业农村部儋州热带作物科学观测试验站,海南儋州 571737
- 3. 华中农业大学园艺林学学院,武汉 430070
- 折叠
摘要
[目的]探究热带雨林2种典型植物(陆均松和青梅)根际真菌群落多样性与组成差异,为保护热带雨林生态系统中珍稀濒危物种的生物多样性提供科学依据.[方法]通过Illumina Miseq高通量测序法对海南热带雨林陆均松和青梅根际真菌群落组成、多样性及功能进行分析.[结果]担子菌门(Basidiomycota)和子囊菌门(Ascomycota)是根际不同生态位间的优势菌门.PCoA结果表明,与生态位相比,物种更能影响陆均松和青梅真菌群落结构,且对根际不同生态位的影响程度不同(根际:R=0.1983,P=0.007;根表:R=0.2622,P=0.004;根内:R=0.7342,P=0.001).在OTU水平上陆均松和青梅真菌群落α多样性差异不显著,但同一树种的根表与根内真菌群落α多样性差异显著(P＜0.05),且与其它生态位相比,根内真菌群落α多样性最低.CCA分析结果表明,环境因子中pH、铵态氮(AN)是影响2个树种根际真菌群落结构的重要环境因子.FUNGuild功能预测结果显示,外生菌根真菌-未定义腐生菌、外生菌根、未定义的腐生菌及寄生真菌-未定义的腐生菌为主要功能类群,且在不同树种中存在差异.[结论]植物种类显著影响根际真菌群落组成,但生态位是在小尺度区域上影响真菌群落多样性的重要因素.pH、AN是驱动陆均松和青梅根际真菌群落组成差异的重要环境因子.
Abstract
[Objective]The diversity and composition of rhizosphere fungal communities of two typical plants(Dacrydium pectinatum and Vat-ica mangachapoi)in tropical rain forest were studied,which provided scientific basis for biodiversity protection of rare and endangered spe-cies in tropical rain forest ecosystem.[Method]The composition,diversity and function of fungal communities in the rhizosphere of D.pecti-natum and V.mangachapoi in Hainan tropical rain forest were analyzed by Illumina Miseq high-throughput sequencing.[Result]Basidiomy-cota and Ascomycota were the dominant phyla among different niches in the rhizosphere.PCoA results showed that compared with niche,species had more influence on the fungal community structure of D.pectinatum and V.mangachapoi and had different effects on different niches in the rhizosphere(rhizosphere:R=0.1983,P=0.007;rhizoplane:R=0.2622,P=0.004;endosphere:R=0.7342,P=0.001).At the OTU level,there was no significant difference in fungal community α diversity between D.pectinatum and V.mangachapoi,but there were significant differences in rhizoplane and endosphere fungal community α diversity of the same tree species(P＜0.05),and the α diversity of endosphere fungal community was the lowest compared with other niches.The results of CCA analysis showed that pH and ammonia nitrogen(AN)were important environmental factors affecting the fungal community structure in the rhizosphere of the two tree spe-cies.The results of FUNGuild function prediction showed that Ectomycorrhizal-Undefined Saprotroph,Ectomycorrhizal,Undefined Saprotro-ph and Fungal Parasite-Undefined Saprotroph were the main functional groups,and there were differences among different tree species.[Conclusion]Plant species significantly affect the composition of rhizosphere fungal communities,but niche is an important factor affecting fungal community diversity in small-scale regions.pH and AN are important environmental factors driving the difference of fungal community composition in rhizosphere between D.pectinatum and V.mangachapoi.
关键词
陆均松/青梅/根际土壤/生态位/真菌/多样性Key words
Dacrydium pectinatum/Vatica mangachapoi/Rhizosphere soil/Niche/Fungi/Diversity引用本文复制引用
基金项目
海南省重点研发项目(ZDYF2024SHF2096)
国家自然科学基金项目(32271603)
海南儋州热带农业生态系统国家野外科学观测研究开放课题(RR1-KLOF202405)
现代农业产业技术体系建设专项(CARS-33-ZP3)
出版年
2024
国家科技期刊平台
NSTL
万方数据