Identification of NAC gene families and analysis of codon usage bias in three Castanea plants
[Objective]The study aimed to identify three NAC gene families of Castanea plants while analyzing codon usage bias.It provided an important theoretical basis for further exploring the function of NAC gene family in chestnut plants.[Method]Using bioinformatics meth-ods,the chromosome position,phyloevolutionary relationships,protein motif,selective pressure,codon usage bias were analyzed in the three Castanea plants.[Result]In the study,333 Castanea NAC gene family members were identified with unequal distribution across different chromosomes.The evolutionary tree analysis revealed that it could be divided into five groups.Protein motif analysis revealed that the five groups all contained the conserved Motif 1-Motif 7.Collinearity analysis showed that it contained strong collinearity within and between the genomes,and most of the collinear gene pairs were undergoing purifying selection pressure.The codon usage bias analysis showed a weak co-don usage bias.Neutral mapping analysis and ENC-plot mapping analysis showed that their codon usage bias was more under natural selective pressure.The optimal codon analysis revealed that the NAC gene families of three Castanea plants together contained 11 optimal codons.For example,CAU,UAA,GUU,etc.Comparing the codon usage frequency with the comparative model plants,Arabidopsis thaliana,Nicotiana tabacum and Solanum lycopersicum were all suitable exogenous receptors for the three Castanea plants.[Conclusion]The study provides a comprehensive understanding of the evolutionary relationships,selection pressures,codon preference of NAC gene family members of Castanea plants.The study lays a theoretical foundation for the later use of molecular biological means to improve the efficiency of NAC gene expression in Castanea plants.
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