Genome-wide identification and expression pattern analysis of WRKY genes family in Erythropalum scandens
[Objective]The study aimed to explore the functional roles of WRKY transcription factors in different tissues and their growth and development in Erythropalum scandens,and to provide theoretical basis for in-depth analysis of molecular regulatory mechanisms of growth and development of E.scandens.[Method]The WRKY gene family across the whole genome of E.scandens was identified by bioinformatics meth-ods,the structure and function of WRKY gene family members were analyzed,and the expression patterns of WRKYs genes in different tissues were analyzed by transcriptome.[Result]A total of 66 EsWRKYs genes were identified,which were distributed on 11 chromosomes.Subcellu-lar localization prediction showed that 62 EsWRKY proteins were located in the nucleus.Phylogenetic analysis divided the WRKY protein family members into three group(Ⅰ-Ⅲ),with 18,44 and 4 members,respectively.Group Ⅱ could be divided into five subcategories(Ⅱa-Ⅱe),with 3,7,18,8 and 8 members,respectively.There were 2-11 exons of 66 EsWRKYs genes.Conservative motif analysis showed that Motif 1 and Motif3 contained WRKY domains.Motif 1 was the most important conserved motif of EsWRKYs protein in E.scandens.The pro-moter region of EsWRKYs gene family members had 19 cis-acting elements related to growth and development,stress response,hormone re-sponse.The expression pattern analysis showed that EsWRKY14,EsWRKY22,EsWRKY29,EsWRKY30,EsWRKY47 and EsWRKY61 might be involved in the growth and development of stems and leaves of E.scandens.Most EsWRKYs genes were significantly down-regulated during kernel development,indicating that these EsWRKYs genes might play a major role in the early stage of kernel development.[Conclusion]The results showe that 66 members of the WRKY gene family members of E.scandens are identified,and their physical and chemical properties were quite different.All of them have WRKY conservative domains.The EsWRKYs genes of E.scandens have different expression patterns in different tissues,and may play an important role in growth and development,stress and secondary metabolism.
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