Evolution analysis of microbial community characteristics in rhizosphere soil of fresh maize at different growth stages
[Objective]This experiment focused on the changes in the composition of soil microorganisms in the root system of maize at differ-ent growth stages,explored the evolution law of soil microbial community composition in maize roots and its coupling relationship with environ-mental factors.[Method]Based on the field experiment of fresh maize,the rhizosphere soil was collected and its physicochemical properties were determined at the pre-sowing stage(PS),jointing stage(JS),tasseling stage(TS),and milky stage(MS)of fresh maize.16S and ITS gene sequencing were used to analyze the characteristics of microbial community structure in the rhizosphere soil of fresh maize at different growth stages.[Result]There were significant differences in the structure and diversity of bacterial communities in the rhizosphere soil of maize at different growth stages,while the composition of fungal communities did not show significant changes at each growth stage.At the jointing stage,with a significant decrease in soil nutrient content,the relative abundance of Proteobacteria increased significantly,while the a-bundance of Ascomycota decreased significantly.During the milk ripening stage,the positive symbiotic relationship between different genera of bacteria in the rhizosphere soil reached its highest level compared to other growth stages,while the positive symbiotic relationship between dif-ferent genera of fungi decreased to its lowest level.The structural equation modeling analysis results showed that there was a positive effect be-tween rhizosphere soil bacterial diversity and soil pH,total phosphorus,and available potassium,while there was a negative effect between fungal diversity and the above indexes.[Conclusion]The structure of soil microbial community has significant changes during the growth peri-od of fresh maize,bacteria are more susceptible to environmental influences compared with fungi,and the changes in microbial community structure with the environment also affect the formation of fresh maize yield.
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