Screening of candidate genes related to pollen abortion of blueberry by transcriptomics
[Objective]The genes related to pollen abortion of blueberry were screened to provide reference for its cloning and related research and breeding.[Method]Illumina NovaSeq 6000 was used to sequence the transcriptomes of pollen from three rabbiteyed blueberry cultivars,'Brightwell(BT)','Powderblue(PB)'and'Premier(PM)',which were derived from the same female parent and had significant differ-ences in pollen viability.The genes related to pollen abortion of blueberry were screened based on the Gene Ontology(GO),Kyoto Encyclo-pedia of Genes and Genomes(KEGG)and Protein family(Pfam).[Result]The pollen viability of'PM'was significantly lower than that of'BT'and'PB',and 2717 and 3208 differentially expressed genes were detected in the comparison combinations of'BT'vs'PM'and'PB'and'PM',respectively.There were 1124,1020 and 1791 differentially expressed genes in the GO,KEGG and Pfam databases of the 2717 differentially expressed genes in the comparison combination of'BT'vs'PM',while the 3208 differentially expressed genes of'PB'vs'PM'were annotated 1350,1144 and 2197 differentially expressed genes in GO,KEGG and Pfam databases,respectively.Further screen-ing of GO and Pfam annotation results of differentially expressed genes revealed that 27 differentially expressed genes were related to pollen a-bortion,of which 22 genes were up-expressed and 5 genes were down-expressed.[Conclusion]The annotation results of GO and Pfam differ-entially expressed genes related to pollen abortion show that pollen development,inflorescence development,sporopollenin biosynthetic process,formation of pollen exine,cell differentiation of anther wall tapetum,positive regulation of cytokinesis,regulation of mitotic spindle or-ganization,brassinosteroid mediated signal process and other pathways are closely related to pollen abortion in blueberry.It can be used as a reference for further exploring the mechanism of pollen abortion and mining the key genes of pollen abortion in blueberry.
万方数据
维普
