Cloning of Hsp70 gene in Sogatella furcifera and its response to abiotic stress
[Objective]The role of heat shock protein Hsp70 gene in the adaptation of Sogatella furcifera to abiotic stresses(insecticide,high/low temperature,and UV-A)was revealed,which provided a reference for the green prevention and control of S.furcifera.[Method]Based on genomic and transcriptomic data,the Hsp70 gene in the S.furcifera was cloned and identified,and its transcription levels treated with insecti-cide,high/low temperature and UV-A stress were measured using RT-qPCR.[Result]A Hsp70 gene was successfully obtained and designat-ed as SfHsp70-1.Its full-length sequence was determined to be 2322 bp,with an open reading frame of 2001 bp,encoding 666 amino acid res-idues,a molecular weight of approximately 72.91 kDa and a theoretical isoelectric point of 5.11.There were three conserved domains of the Hsp70 family proteins in SfHsp70-1,with a conserved C-terminal endoplasmic reticulum Hsp70 sequence'HDEL'.The sublethal concentra-tion of thiamethoxam showed a significant dose effect,and LCio significantly induced the transcriptional level of the SfHsp70-1 gene to 2.59 fold that of the control(acetone treatment),whereas LC25 concentration stress showed no significant impact.Additionally,temperature stress at 10 ℃(30 min),20 ℃(30,60 min),and 40 ℃(10,30,60 min)significantly induced the transcriptional levels of the SfHsp70-1 gene by 77.24％,62.00％,85.89％,138.96％,75.67％and 61.10％compared to the control(25 ℃).UV-A(300 μW/cm2)stress signifi-cantly inhibited the transcriptional levels of the SfHsp70-1 gene,with a reduction of 87.85％,88.74％,91.91％,89.78％,93.04％and 91.16％,respectively,compared to the control(0 min exposure),and the expression level showed a gradually downward trend with the ex-tension of treatment time.[Conclusion]The overexpression of the SfHsp70-1 gene contributes to the S.furcifera adaptation to thiamethoxam and high/low temperature stress.These findings provide a foundation for further investigating the role of the Hsp70 gene in the adaptation of the S.furcifera to abiotic stress and providing a reference for the green prevention development and control technology for S.furcifera.
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