Identification and expression analysis of sorghum LBD gene family under drought stress during seedling stage
[Objective]The paper aimed to investigate the functional role of the LBD gene family in regulating drought stress in sorghum,and provide a basis for the use of Sorghum bicolor molecular breeding.[Method]The bioinformatics method was used to predict and analyze the physicochemical characteristics,chromosome distribution,phylogeny,gene structure,conserved motifs and cis-regulatory elements of the SbLBD family of sorghum.[Result]32 SbLBD genes were identified from sorghum,which were distributed unevenly on 9 chromosomes.These SbLBD genes were divided into two groups:Class Ⅰ and Class Ⅱ.25 SbLBD genes in Class Ⅰ contained CX2CX6CX3C,GAS and LX6LX3LX7L conserved domains.7 SbLBD genes in Class Ⅱ contained CX2CX6CX3C and GRA conserved domains.The homology between sorghum LBD protein and monocotyledonous plants was greater than that of dicotyledonous plants.Analysis of cis regulatory elements revealed that the number of light responsive elements(G-box)and abscisic acid responsive element(ABRE)in the SbLBD promoter sequence were the highest,followed by methyl jasmonate responsive element(CGTCA-motif)and anaerobic induced responsive element(ARE).In addi-tion,low-temperature responsive element(LTR)and drought induced element(MBS)were detected.Expression analysis showed that ex-pression levels of LBD genes in Class Ⅱ and SbLBD24 in Class Ⅰ were higher in those of different tissues.The transcriptome data of LBD genes were analyzed under PEG6000 treatment,the expression levels of SbLBD6 and SbLBD26 of drought tolerant sorghum were down-regulated and up-regulated respectively.[Conclusion]There were differences in the conserved sequences of SbLBD genes among Class Ⅰ and Class Ⅱ groups.SbLBD genes could respond to various stress conditions such as low temperature,drought,light intensity and photoperiod.There was tissue expression specificity of SbLBD genes.SbLBD6 and SbLBD26 may play important roles in drought stress resistance of sorghum.
万方数据
维普
