Identification of CCD gene family and its expression analysis in response to salt and flooding in Salix matsudana
[Objective]Carotenoid cleavage dioxygenases(CCDs)catalyze the breakdown of various carotenoids into smaller molecules,a process essential for plant growth,development and response to abiotic stresses.A thorough understanding of the biosynthesis and degradation mechanisms of carotenoids is crucial for precisely regulating their levels within plants.[Method]We selected the indigenous Chinese variety of Salix matsudana as the experimental material and utilized its whole-genome database to identify and analyzed members of the SmCCD fami-ly.The research included constructing a phylogenetic tree,analyzing cis-acting elements,exploring conserved motifs and gene structures,con-ducting collinearity analysis,building protein interaction networks,performing Gene Ontology(GO)annotation analysis and transcriptome a-nalysis.[Result]The study successfully identified 17 CCD genes from the S.matsudana genome and conducted an in-depth analysis of their characteristics and expression patterns.These SmCCD members were classified into two subfamilies:SmCCD and SmNCED.The SmCCD sub-family was further divided into categories such as SmCCD1,SmCCD4,SmCCD7 and SmCCD8.Collinearity analysis revealed that segmental duplication was a key factor leading to the amplification of SmCCD genes.By comparing the gene collinearity between willow,Arabidopsis,rice and poplar,we found a higher number of gene pairs among willow,poplar and Arabidopsis than between willow and rice,supporting the closer evolutionary relationship between willow and other dicotyledonous plants such as poplar.Additionally,transcriptome analysis indicated signifi-cant changes in the expression levels of SmCCD genes in S.matsudana under salt and waterlogging stresses,suggesting their potential key role in the plant's adaptation to environmental stress.[Conclusion]The study reveals the diversity of the SmCCD gene family and their expression patterns under environmental stress,providing new molecular evidence for understanding the adaptability and evolutionary relationships of wil-low.These findings are instrumental in guiding the breeding and ecological restoration of willow to address the challenges of environmental change.
万方数据
维普
