Effects of tillage methods with different crop rotation patterns on soil bacteria in wheat's season in Huaihe river basin
[Objective]The study aimed to eluvidate the response characteristics of soil bacterial community composition and species diversity to conservation tillage under different crop rotation systems,and provide theoretical basis for the efficient rotation and agricultural soil sustain-able utilization.[Method]The study selected Huaihe river basin in Henan as a model area,high-throughput sequencing technology was used to sequence and analyze the wheat's seasonsoil bacteria at the 0-5 and 5-10 cm layer under rice-wheat rotation and maize-wheat rotation systems,and to study the effects of conservation tillage on soil bacterial communities.[Result]There were 45 phyla,126 classes,189 orders and 229 families of soil bacteria detected in the study.The dominant bacterial phyla were mainly Proteobacteria,Acidobacteria,Bacteroidetes and Gemmatimonadetes,with relative abundance of 35.65％ ,24.32％ ,10.12％ and 6.76％ ,respectively;The impact of tillage methods on soil bacteria had a significant hierarchical effect.Conservation tillage significantly increased the number of bacterial gene sequences,dominant bacterial sequences,dominant bacterial functional groups,and total enzyme abundance in the 0-5 cm soil layer.The increase effect was higher in maize-wheat rotation soil than in rice-wheat rotation soil,while rotary tillage mainly promoted the increase of bacterial quantity and functional enzymes in the 5-10 cm soil layer;Predicted functions analysis showed 6 classes of primary classification,and 47 bacterial func-tional pathways of secondary classification.Among which the primary pathway,with the highest proportion of metabolic functional groups(73.13％ -73.79％ ),followed by genetic information process functional groups(8.51％ -10.73％ ).[Conclusion]Maize-wheat rotation is beneficial for improving sequence number,functional group,and total enzyme abundance of soil bacteria.Conservation tillage mainly affects the dominant bacterial population in the 0-5 cm layer,while rotary tillage reduces the hierarchical differences of soil bacterial population.Research on the impact of tillage methods on soil bacteria should also consider the effects of crop rotation and soil layers.
万方数据
维普
