Physiological and biochemical reactions of potatoes in response to infection by Pectobacterium atrosepticum
[Objective]Potato black shin disease caused by(Pectobacterium atrosepticum,Pa)was one of the major diseases affecting potato yield and quality.The study aimed to compare the defense responses of different resistant varieties to elucidate how resistant potatoes regulate the activity of defense enzymes to resist the attack of pathogens.[Method]The disease-resistant variety Qingshu No.9 and susceptible variety Leshu No.1 were inoculated with P.atrosepticum in the greenhouse.The enzyme activities of SOD,CAT,POD,PPO,PAL and β-1,3-glu-canase,as well as the changes of lignin and total phenol contents at different time points(1,2,3,4 days)after injection were analyzed.[Re-sult]After inoculation with Pa,the activity of CAT,SOD,PPO and PAL enzymes,and the content of lignin in resistant varieties were significantly higher than those in control and were considerably higher than those in susceptible varieties.Susceptible varieties showed higher POD activity at the initial stage of infection.Still,with the increase in infection time,the growth rate and peak value of POD activity between 2 and 3 days were lower than those of resistant varieties.Although β-1,3-glucanase activity and total phenol content did not show significant differences in resistant varieties,the changing trend existing between the two varieties differed from 1 to 4 days after injection.[Conclusion]The activity levels of SOD,CAT,POD,PPO,PAL,lignin and total phenol in resistant varieties are significantly different,and the change rate of physiological indexes of re-sistant varieties after inoculation with pathogenic bacteria is considerably higher than that of susceptible varieties.
Potato black shankDefense enzyme activityLigninTotal phenols
万方数据
维普
