Identification and biological characteristics of pathogen causing anthracnose on fruit of Annona atemoya in Guangxi
[Objective]The study aimed to clarify the classification and biological characterization of pathogen causing anthracnose on fruit of Annona atemoya in Guangxi,so as to provide theoretical basis for the scientific control of the disease in the field.[Method]Tissue isolation was used to isolate the collected samples of anthracnose fruit of A.atemoya,and Koch's rule was used to determine the pathogenicity of the isolated strains.The pathogen was identified by combining morphological characteristics and molecular biology.The tandem phylogenetic tree was constructed using ITS,GAPDH,CAL,ACT,CHS-1 and TUB2 sequences.Biological characteristics were determined by colony growth rate method.The fungicides carbendazim,chlorothalonil,difenoconazole,ovraclostrobin,prochloraz,mancozeb,thiophanate-methyl and te-buconazole were selected for efficacy testing on the pathogen.[Result]Five strains of the pathogen were isolated from anthracnose fruits of A.atemoya in Guangxi,and all strains were morphologically consistent.Representative strains FLZGB3 and FLZ5 were selected for molecular biology determination,and the constructed phylogenetic tree showed that strains FLZGB3 and FLZ5 were also clustered with Colletotrichum siamense on the same branch.Combining the results of morphological characteristics and phylogenetic analysis,the pathogen was identified as C.siamense.The results of biological characterization showed that the most suitable temperature for mycelial growth of strain FLZGB3 was 28.0 ℃,the optimal pH was 8,the optimal light condition was continuous darkness,the optimal medium was Czapek medium,the optimal carbon was glucose and the optimal nitrogen was yeast extract.Prochloraz and carbendazim had strong inhibitory effect on mycelial growth of strain FLZGB3,the inhibition rates were 100.00％ and 97.16％ respectively.[Conclusion]The pathogen of anthracnose on A.atemoya fruits in Guangxi was identified as C.siamense.Miconazole and carbendazim can greatly inhibit the growth of the pathogen,which can be promoted and used in the prevention and control of anthracnose on A.atemoya fruits in Guangxi.
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