Transcriptomic analysis of responding to Fusarium oxysporum infection in Gerbera jamesonii
[Objective]The changes in gene expression of Gerbera jamesonii roots under Fusarium oxysporum infection are proved to explore the related genes and molecular mechanism of signaling pathway of G.jamesonii roots to resist bacterial stress and provide a theoretical refer-ence for control of root rot in G.jamesonii.[Method]The transcriptome of the healthy and infected G.jamesonii roots by F.oxysporum for 96 hours was sequenced by transcriptome sequencing technology to screen expressed genes(DEGs).Their GO function was annotated and en-richment of KEGG metabolic pathway was analyzed.Finally,the reliability of transcriptome sequencing results was verified by real-time fluo-rescence quantitative PCR.[Result]Total 10 218 DEGs including 7273 up-regulated genes and 2945 down-regulated genes were obtained from the infected G.jamesonii roots.There were 3401 DEGs belonging to 72 transcription factor families.The MYB and AP2/EFR families had the highest number of transcription factors,followed by C2H2,bHLH,NAC and C3H families.The enrichment results showed that 53 functional groups were annotated from the DEGs and the DEGs related to redox processes,cytoplasmic sol,cell division sites,membrane com-position,and metal ion binding were more abundant.The enrichment analysis of KEGG metabolic pathway showed that DEGs were enriched in the biosynthetic pathways of carbohydrate metabolism,energy metabolism,lipid metabolism and amino acid metabolism.The DEGs of 3 carbo-hydrate metabolic pathways were up-regulation mostly.Most DEGs in pathways of photosynthesis and photosynthesis-antenna protein were down-regulation.49 differentially expressed genes were enriched in theα-linolenic acid metabolism pathway,and 4 lipoxygenase(LOX2S)genes and 2 hydroperoxide dehydratase(AOS)genes were down-regulation.[Conclusion]The members of transcription factor families in-clude MYB,AP2/EFR,C2H2,bHLH,NAC and C3H.DEGs regulating photosynthesis,carbohydrate metabolism,lipid metabolism and so on may play an important role in response to root rot stress in G.jamesonii.
万方数据
维普
