Response of tobacco rhizosphere bacteria under different treatment of organic and microbial fertilizer
[Objective]The study aimed to clarify the effect of bio-organic and microbial fertilizers on tobacco rhizosphere bacterial community structure,identify key response bacterial communities,and explore the dynamic response process of tobacco rhizosphere bacteria under the treatment of bio-organic and microbial fertilizer.[Method]The study focused on the response mechanism of tobacco rhizosphere bacteria to chemical fertilizers,bio-organic fertilizers and plant growth promoting microbes(PGPMs).The Illumina MiSeq high-throughput sequencing technology was used to sequence the 16S rRNA genes V3-V4 of tobacco rhizosphere bacteria under different treatments.After clustering of op-erational taxonomic units(OTU),we analyzed the diversity of bacterial communities among treatments.Then we established models through machine learning methods as well as bacterial co-occurrence network to analyze the key regulatory bacteria in response to different fertilization treatments.[Result]Compared to chemical fertiliiers,the application of bio-organic and microbial fertilizer significantly increased the abun-dance of tobacco rhizosphere bacteria,but decreased the uniformity of the bacterial distribution.Based on the model,15 characteristic bacteri-al genus associated with fertilization treatment were further identified.Among them,Pantoea and Stenotrophomonas were indicator bacteria un-der PGPRs.The co-abundance network structure of rhizosphere bacteria changed from a loose and diverse state under chemical fertilizer treat-ment to a tightly and complex regulated state under microbial fertilizer treatment.Under PGPRs,the bacterial regulation enhanced and it was closely connected with the surrounding flora.[Conclusion]Bio-organic and microbial fertilizers significantly change the tobacco rhizosphere bacterial community structure and rhizosphere bacterial response network.The key regulatory bacteria,such as Pantoea and Stenotroph-omonas,have the value of further purification,culture and commercial development.
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