Diversity and functional prediction of soil bacterial communities across different elevations in tropical rainforest of Diaoluo Mountain
[Objective]The study aimed to explore the distribution patterns and environmental driving factors of soil bacterial community characteristics at different elevations in tropical forests,and to provide data support for the theoretical study of microbial biogeography.[Meth-od]In the study,the area surrounding Diaoluo Mountain in the Hainan Tropical Rainforest National Park served as the research site.Using high-throughput sequencing technology of 16S rDNA amplicons,the composition,diversity and function of soil bacterial communities were an-alyzed along the elevation gradient.Correlation analysis was conducted to explore the environmental driving factors.[Result]The diversity of soil bacterial communities decreased monotonically with increasing elevation.Alphaproteobacteria,Gammaproteobacteria,Actinobacteria and Flavobacteriaceae were dominant bacterial groups.Their relative abundances showed trends of monotonic increase,initial increase then de-crease,and initial decrease then increase with elevation,respectively.Correlation analysis indicated that bacterial community composition was significantly correlated with soil pH,organic matter,total potassium,ammonium nitrogen,available phosphorus and available potassium.PICRUSt functional prediction indicated that amino acid transport and metabolism,energy production and conversion,translation/ribosomal structure,biotransformation,and cell wall/membrane/envelope biogenesis were the dominant COG functions.Genes involved in nitrogen cyc-ling,such as glnA/GLUL,gltD,cynT/can,gltB,ncd2/npd were predominant.The relative abundances of nirK,arcC,cah,norB,nirS and nrfH were significantly higher at mid-elevations,while GLU/gltS and nrtD/cynD were higher at low elevations.[Conclusion]Soil pH and total po-tassium are key driving factors for the distribution characteristics of bacterial communities along the elevation gradient,providing data support and theoretical basis for the scientific conservation and rational utilization of tropical forest soil microbial resources.
万方数据
维普
