Accumulation mechanism of dihydromyricetin in Ampelopsis grossedentata and A.cantoniensis
[Objective]By analyzing and comparing the transcriptome sequences of Ampelopsis grossedentata and A.cantoniensis,the genes re-lated to the accumulation of dihydromyricetin(DHM)were excavated,which provided new ideas and methods for exploring the high DHM ac-cumulation mechanism of A.grossedentata.[Method]The leaves of A.grossedentata and A.cantoniensis were used as materials,and the DHM content of the two materials was analyzed by high performance liquid chromatography(HPLC).RNA-Seq and qRT-PCR techniques were used to analyze the expression patterns of genes related to DHM metabolic pathway.[Result]The difference in DHM content in the young leaves of A.grossedentata and A.cantoniensis could be as high as 15 times,with DHM content of 336.8 mg/g in young leaves of A.grossedent-ata and 20.8 mg/g in A.cantoniensis.Through differential expression screening of the transcriptome,a total of 14 914 differentially expressed genes(DEGs)were obtained,and a large number of DEGs were significantly enriched in pathways such as ribosome,glycolysis/gluconeogen-esis,carbon fixation in photosynthetic organisms,phenylpropanoid biosynthesis and flavonoid biosynthesis.Enrichment results showed that there were significant differences in the genes involved in flavonoid synthesis and catabolism between A.grossedentata and A.cantoniensis.The results of GO enrichment analysis showed that DEGs were highly involved in the primary metabolic process of plants,especially the synthesis and transport of ribosome-related enzymes.In the study,it was found that the expression levels of AgCHS,AgCHI,AgF3'H and AgF3'5'H genes were high and significantly upregulated,suggesting that these genes were key genes in the flavonoid biosynthesis pathway of A.grossed-entata DHM accumulation.In addition,in the DHM degradation pathway,the expression of the CYP81E gene was significantly downregulat-ed,suggesting that the low expression of this gene might be one of the reasons for the high accumulation of DHM.In addition,in the DHM degradation pathway,the expression of the CYP81E gene was significantly downregulated,suggesting that the low expression of this gene might be one of the reasons for the high accumulation of DHM.[Conclusion]The upregulation of AgCHS,AgCHI,AgF3'H and AgF3'5'H genes,and the downregulation of the AgCYP81E gene,can promote the accumulation of dihydromyricetin in the leaves of A.grossedentata.The study provides new insights and approaches for regulating the accumulation of secondary metabolites in medicinal plants and cultivating high-quality medicinal materials.
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