Identification and Localization of QTL Based on a Rice Large Grain Chromosome Segment Substitution Line Z29
As one of the three elements of rice yield,grain size has an important impact on yield.It is im-portant to explore new genes for grain development.In this study,a rice chromosomal segment substitu-tion line Z29 was selected from a cross with Nipponbare as the recipient and the self-bred indica rice restor-er line R225 as the donor parent.Z29 contains 10 substitution segments from R225 with an average substi-tution length of 2.90 Mb.Both grain length and width of Z29 were increased significantly,showing a large grain phenotype,and its grain enlargement was caused by the highly significant increase in the number and size of glume cells.A total of eight grain shape-related QTLs were detected in the secondary F2 population constructed by crossing Z29 with Nipponbare.Fourteen secondary chromosome segment substitution lines were selected from F3 population using MAS method,including four single segment substitution lines,five double segment substitution lines,two triple segment substitution lines and three quadruple segment substitution lines.These results lay the foundation for cloning grain shape-related QTLs and elucidation of the molecular mechanism,and provide resources for molecular design breeding.
NETL
NSTL
万方数据
