基于水稻大粒染色体片段代换系Z29的鉴定及QTL定位
Identification and Localization of QTL Based on a Rice Large Grain Chromosome Segment Substitution Line Z29
刘金艳 1张朔语 1宗涵颖 1陈文博 1韦秘 1吴如会 1母建妍 1张记超 1凌英华 1张长伟 1何光华 1赵芳明 1张婷1
作者信息
- 1. 西南大学 农学与生物科技学院/南方山地农业教育部工程中心,重庆 400715
- 折叠
摘要
千粒质量作为水稻产量的三要素之一,对产量的影响较大,其中千粒质量主要受水稻粒型的影响,因此挖掘新的粒型基因在生产中具有重要的意义.研究选育到1个以日本晴为受体亲本、自育优良籼稻恢复系R225为供体亲本的水稻染色体片段代换系(CSSL)Z29.Z29含有来自R225的10个代换片段,平均代换长度2.90 Mb.Z29粒长和粒宽均极显著增加,表现为大粒表型,且其籽粒变大是由颖壳细胞数量极显著增多、增大引起.利用日本晴与Z29杂交构建的次级F2 群体进行QTL定位,共检测到8个粒型相关QTL.进一步利用 MAS法在F3 群体中选育出14个次级染色体片段代换系,包括4个单片段代换系、5个双片段代换系、2个三片段代换系和3个四片段代换系.结果可为目的粒型相关QTL克隆和分子机制解析奠定基础,为分子设计育种提供资源.
Abstract
As one of the three elements of rice yield,grain size has an important impact on yield.It is im-portant to explore new genes for grain development.In this study,a rice chromosomal segment substitu-tion line Z29 was selected from a cross with Nipponbare as the recipient and the self-bred indica rice restor-er line R225 as the donor parent.Z29 contains 10 substitution segments from R225 with an average substi-tution length of 2.90 Mb.Both grain length and width of Z29 were increased significantly,showing a large grain phenotype,and its grain enlargement was caused by the highly significant increase in the number and size of glume cells.A total of eight grain shape-related QTLs were detected in the secondary F2 population constructed by crossing Z29 with Nipponbare.Fourteen secondary chromosome segment substitution lines were selected from F3 population using MAS method,including four single segment substitution lines,five double segment substitution lines,two triple segment substitution lines and three quadruple segment substitution lines.These results lay the foundation for cloning grain shape-related QTLs and elucidation of the molecular mechanism,and provide resources for molecular design breeding.
关键词
水稻/染色体片段代换系/粒型/QTL定位/产量Key words
rice(Oryza sativa L.)/chromosome segment substitution line/grain shape/QTL localiza-tion/yield引用本文复制引用
出版年
2024
NETL
NSTL
万方数据