Phenotypic Characterization and Gene Mapping of a Presenescing Leaf Mutant psl4(t)in Rice(Oryza sativa L.)
Early leaf senescence greatly affects grain yield and quality in rice.It is of great significance to mine genes related to rice leaf premature senescence and analyze their molecular mechanisms to improve rice yield.A presenescing leaf mutant psl4(t)was obtained from the hybrid offspring of Fuhui9802 and LR in this study.The psl4(t)mutant exhibited a normal phenotype up to the L6 stage.Nevertheless,from seventh leaf to flag leaf,each leaf gradually senesced from leaf tip to middle of leaf.The chloroplast development of leaves was inhibited,the size of chloroplasts became smaller,the content of photosynthetic pigments decreased,which leads to the advanced leaf senescence.The results of agronomic trait analysis showed that compared with the wild type,the psl4(t)mutant had no significant changes in panicle length,effective tiller number and seed width,while plant height,grain number per panicle,seed setting rate and thousand kernel weight significantly reduced.Genetic analysis revealed that the premature senescence trait of psl4(t)mutant was controlled by a single recessive gene.The target gene was mapped in a 38.5 kb re-gion on chromosome 4 between marker RM17004 and RM17006 through gene mapping.This study pro-vides a basis for psl4(t)gene cloning and functional analysis,and molecular mechanism of premature leaf senescence.
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