Studies on the Damage of the Small Intestinal Barrier in Mice by Chronic Restraint Stress
The gut barrier consists of mechanical,immune,chemical and biological barriers,which plays an important role in maintaining the homeostasis of the intestinal lumen and the integrity of the intestinal epithelium.Firstly,hormone level tests and open field test were used to verify whether the mouse chronic restraint stress model was constructed successfully.Subsequently,conventional histological staining,im-munohistochemistry,TUNEL immunofluorescence and ELISA were used to investigated the effects of chronic restraint stress on mice gut barrier.Our results showed that compared with the control group,chronic restraint stress significantly increased plasma NE and CORT levels(p<0.05),decreased the height of small intestinal villi(V,p<0.05),increased the depth of crypts(C,p<0.05),and decreased the V/C ratio(p<0.05),the positive expression of the tight junction protein ZO-1(p<0.01),the num-ber of small intestinal goblet cell and endothelial mononuclear lymphocytes(p<0.01),suggesting that chronic restraint stress caused intestinal damage and immune suppression in mice.Further studies revealed that chronic stress significantly decreased PCNA positive expression(p<0.01),while increased apoptotic cells numbers(p<0.01)in the small intestine.The plasma levels of TNF-α,IL-1β,and IL-18 were sig-nificantly increased(p<0.01),while IL-10 was significantly decreased(p<0.01)than that of control group.In addition,the results of plasma oxidation-antioxidant indexes showed that chronic restraint stress significantly increased MDA content(p<0.05)and decreased T-AOC,SOD,CAT and GSH-Px levels(p<0.05)in mice.This study demonstrated that chronic restraint stress can inhibit cell prolifera-tion and damage the intestinal mucosal barrier function,aggravate inflammatory reactions by inducing ox-idative stress in mice.
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