Dynamic Response of Chlorophyll Fluorescence in Microalgae Driven by Streptomycin
The response of Raphidiopsis raciborskii and Scenedesmus quadricauda on streptomycin was determined by OJIP chlorophyll fluorescence kinetics to explore the sensitivity of microalgae to streptomy-cin.The results showed that streptomycin significantly facilitate the DI0/RC,ABS/CS0 and ABS/RC in Raphidiopsis raciborskii and Scenedesmus quadricauda,inhibited TR0/RC,and markedly reduced the photosynthetic oxygen evolution and driving force in Raphidiopsis raciborskii.The specific growth rates of Raphidiopsis raciborskii(0.05～1.0 mg/L)and Scenedesmus quadricauda(1.0～20.0 mg/L)in strep-tomycin treatment group was 0.74～0.25 and 1.19～0.51 times as much as that of control(0.00 mg/L),respec-tively.This study found that the malondialdehyde and superoxide dismutase activities were decreased sig-nificantly,and catalase activity was increased in Raphidiopsis raciborskii with increase of streptomycin concentration.Malondialdehyde,superoxide dismutase and catalase activities in Scenedesmus quadri-caudawere not significantly affectedby streptomycin.Therefore,our results suggested that cayanobacteria was more sensitive to streptomycin than green algae,the main reason is due to the significant difference on energy distribution between Raphidiopsis raciborskii and Scenedesmus quadricauda.
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