An Analysis of the Abundance of Fatty Acids in Silkworm Female Pupa and Ovary
The study utilized gas chromatography analyzing the abundance of fatty acids in the bodies and o-varies of female silkworm pupae.The results revealed that the variation range of fatty acid abundance in the pupal body was small at first six days of pupation but increased from the sixth to ninth day.Overall,the main fatty acids exhibited a decreasing trend,while the trace fatty acids showed an increasing trend.The variation range of fatty acid abundance in the ovaries decreased with development.Different main fatty acids exhibited diverse patterns and directions of change,with an increase in unsaturated fatty acid abun-dance and a decrease in saturated fatty acid abundance.The abundance of trace fatty acids significantly de-creased with development,indicating a less association between trace fatty acids and female reproduction.The differences in fatty acid abundance between the pupal body and ovaries gradually diminished with de-velopment.By the sixth day of pupation,most of fatty acids in the ovaries differed significantly from those observed during the initial pupation stage,but were similar to those in the pupal body,suggesting the de-pendence of fatty acid accumulation in ovary on the pupal body fatty acids,which is likely related to ovari-an development and vitellogenesis.In the later stages of pupation,the highest abundance of α-Lindenic acid fatty acid exhibited a decrease,while the second highest abundance of Oleicacid fatty acid significantly increased,which may be associated with eggshell formation and oocyte maturation.Linoleic acid displayed the greatest difference on the sixth day of pupation,with a significantly higher abundance in the ovaries than in the pupal body,indicating the specificity of accumulation of fatty acids in ovary during development and oogenesis.These results provide important clues for further investigating the mechanisms underlying the role of fatty acids in female reproduction of silkworms and other insects.
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