The study of nobiletin from Citri Reticulatae Pericarpium on Anti-inflammatory by inhibiting IL-23/IL-17 axis
Objective To study the anti-inflammatory effect and mechanism of on LPS-induced mouse macrophages.Methods CCK-8 method was used to detect the cytotoxicity of nobiletin on mouse macrophages and to determine the safe dosing concentration.LPS was used to stimulate mouse macrophages to construct a cellular inflammation model,and 5-80 μmol/L of nobiletin was added for 24 h.The NO content in the cell supernatant was detected by the Griess method and the secretion levels of pro-inflammatory factors(TNF-αand IL-6)in mouse macrophages were detected by ELISA.The mRNA expressions of TNF-α,IL-6,IL-23 and IL-17A in experimental cells were tested by RT-PCR.Results CCK-8 results showed that the difference in cell viability was not statistically significant(P＞0.05)when the concentration of nobiletin was at 80 μmol/L and below,and the NO content in the cell supernatant was significantly elevated(P＜0.01)after LPS stimulation,which indicated that the cellular inflammation model was successfully constructed.Compared with the LPS group,nobiletin treatment significantly reduced the NO content in the cell supernatant(P＜0.01),the secretion of cellular pro-inflammatory factors(TNF-α and IL-6)(P＜0.01),and the expression levels of TNF-α,IL-6,IL-23,and IL-17A mRNA(P＜0.01)in a dosage-dependent manner.Conclusion Nobiletin alleviates the LPS-induced inflammatory response of mouse macrophages through regulating the inflammatory axis of IL-23/IL-17 and inhibiting the production of pro-inflammatory factors(TNF-α and IL-6).
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