Immobilization of Lipase from Aspergillus oryzae on ZIF-8 and Catalytic Preparation of Biodiesel
Biodiesel is a sustainable and environmentally friendly bio-liquid fuel,and finding an efficient method for lipase immobilization to produce biodiesel enzymatically is a priority.Metal-organic frameworks,known for their high porosity,adjustable pore size,ease of functionalization,and modification,are excellent porous carrier materials.In this study,the metal-organic framework ZIF-8 was used as a carrier to immobilize lipase from Aspergillus oryzae(AOL)via co-precipitation,resulting in the formation of AOL@ZIF-8 with a nanoflower structure.Biodiesel was synthesized using soybean oil as raw material with AOL@ZIF-8,free AOL enzyme,and commercial immobilized enzyme Novozym 435 as catalysts.The production process was optimized,and the catalytic effects were subsequently compared.Under optimal conditions,the yield of biodiesel catalyzed by AOL@ZIF-8 reached 94.08%.Its activity and reusability were significantly better than the free enzyme and comparable to the commercial immobilized enzyme Novozym 435.
lipase from Aspergillus oryzaemetal-organic frameworksZIF-8immobilizationbiodiesel
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