芹菜素上调Quaking表达减轻棕榈酸诱导心肌细胞凋亡
Apigenin Alleviates Palmitic Acid-induced Cardiomyocytes Apoptosis by Upregulating Quaking Expression
杨雅元 1阚洪爽 1贾宏玉 1杨晓松 2欧阳昌汉 2李莹莹 3王淼4
作者信息
- 1. 湖北科技学院医学部药学院,湖北 咸宁 437100
- 2. 湖北科技学院糖尿病心脑血管病变湖北省重点实验室
- 3. 湖北科技学院核技术与化学生物学院
- 4. 咸宁市中心医院/湖北科技学院附属第一医院心血管内科
- 折叠
摘要
目的 探讨芹菜素(APi)在棕榈酸(PA)诱导H9C2 心肌细胞凋亡中的作用与机制.方法 APi(或QKI过表达)干预PA诱导H9C2 心肌细胞凋亡;Western blot检测细胞中蛋白表达;Fluo-4AM测定细胞内钙离子浓度.结果 10μmol/L APi抑制PA诱导H9C2 心肌细胞中Cleaved-caspase 3 蛋白表达的效果最佳,随着APi浓度的继续增加,其抑制效果逐渐减弱;APi的干预能够显著抑制PA诱导的QKI、SERCA2α、RyR2 蛋白表达下调和Cleaved-caspase 3 蛋白表达的增加;同样,QKI的过表达亦显著抑制PA诱导的SERCA2α、RyR2 蛋白表达下调和Cleaved-caspase 3 蛋白表达的增加;APi(或QKI过表达)减轻PA诱导H9C2 心肌细胞内钙离子浓度的增加.结论 APi促进心肌细胞中QKI的表达,从而维持细胞内Ca2+动态平衡,改善PA诱导的心肌细胞凋亡.
Abstract
Objective To explore the role and mechanism of apigenin(APi)in palmitate acid(PA)-induced apoptosis in H9C2 cardiomyocytes.Methods APi(or QKI overexpression)intervened in PA-induced apoptosis of H9C2 cardiomyocytes;West-ern Blot was performed to detect protein expression in cells;Intracellular calcium concentration was determined by Fluo-4AM.Results 10μmol/L APi had the best effect on PA-induced Cleaved-caspase 3 protein expression in H9C2 cardiomyocytes,and the inhibitory effect was gradually weakened as the concentration of APi increased.The intervention of APi significantly inhibited the PA-induced down-regulation of QKI,SERCA2α,RyR2 expression and up-regulation of Cleaved-caspase 3 protein expression.Simil-ary,overexpression of QKI significantly inhibited the PA-induced down-regulation of QKI,SERCA2α,RyR2 expression and up-reg-ulation of Cleaved-caspase 3 protein expression.The intervention of APi(or QKI overexpression)alleviated the PA-induced in-crease in calcium concentration in H9C2 cardiomyocytes.Conclusion APi promotes the expression of QKI in cardiomyocytes,there-by maintaining intracellular Ca2+dynamic balance and improving PA-induced cardiomyocyte apoptosis.
关键词
芹菜素/QKI/棕榈酸/钙离子/细胞凋亡Key words
Apigenin/QKI/Mitochondria/Calcium ion/Apoptosis引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
万方数据