姜黄素对糖尿病小鼠肺纤维化的调控作用及机制研究
Regulatory Effects and Mechanisms of Curcumin on Pulmonary Fibrosis in Diabetic Mice
黎双炼 1付晨禄 1管志威 1刘超2
作者信息
- 1. 湖北科技学院医学部药学院,湖北 咸宁 437100;湖北科技学院糖尿病心血管病变湖北省重点实验室
- 2. 湖北科技学院糖尿病心血管病变湖北省重点实验室
- 折叠
摘要
目的 探讨姜黄素(Cur)对糖尿病(DM)小鼠肺纤维化的改善作用及其机制.方法 将 50 只雄性SPF级C57BL/6 小鼠随机分为正常组(Con组,n=10)、姜黄素组(Cur组,n=10)、糖尿病模型组(DM组,n=12)和糖尿病姜黄素处理组(DM+Cur组,n=14).DM组与DM+Cur组小鼠先给予高糖高脂饲料喂养4 周后,小剂量腹腔注射链脲佐菌素[STZ,50mg/(kg·d)]建立糖尿病小鼠模型,1 次/d,连续 5d,随机血糖 16.7mmol/L则为造模成功标准.造模成功后,Cur组与DM+Cur组灌胃给予Cur,200mg/(kg·d),连续 16 周后处死小鼠,取肺组织,HE染色法、Masson染色及Sirius Red染色观察各组小鼠肺组织病理学改变、炎细胞浸润及胶原沉积情况;Western blot法检测各组小鼠肺组织中TGF-β1、p-Smad3、p-P38MAPK、Collagen Ⅲ蛋白表达情况.结果 与Con组相比,DM组小鼠肺组织呈明显纤维化改变,肺组织中TGF-β1、p-Smad3、p-P38MAPK、Collagen Ⅲ蛋白表达明显升高(P＜0.01).给予Cur处理后,DM小鼠肺组织纤维化得到明显改善,肺组织中TGF-β1、p-Smad3、p-P38MAPK、Collagen Ⅲ蛋白表达均有所降低(P＜0.05).结论 Cur通过降低纤维化相关蛋白Collagen Ⅲ的表达明显改善STZ诱导的DM小鼠肺组织纤维化,其作用机制可能与Cur抑制肺组织中TGF-β1/Smad/P38MAPK信号通路激活有关.
Abstract
Objective To investigate the improving effect of curcumin(Cur)on pulmonary fibrosis in diabetic(DM)mice and its mechanism.Methods 50 male SPF grade C57BL/6 mice were randomly divided into a normal group(Con group,n=10),a curcumin group(Cur group,n=10),a diabetic model group(DMgroup,n=12)and a diabetic curcumin treatment group(DM+Cur group,n=14).The mice in DMgroup and DM+Cur group were first fed with a high-sugar and high-fat diet for 4 weeks,and then a small dose of streptozotocin[STZ,50mg/(kg·d)]was intraperitoneally injected to establish a diabetic mouse model,once a day for 5 consecutive days.The successful modeling standard was 16.7mmol/L.After successful modeling,the Cur group and the DM+Cur group were gavaged with Cur at200mg/(kg·d).After 16 consecutive weeks,the mice were killed and the lung tissues were obtained.HE staining,Masson staining and Sirius Red staining were used to observe the pathological changes,inflam-matory cell infiltration and collagen deposition in the lung tissues of various groups of mice.Western blot was used to detect the ex-pressions ofTGF-1,p-Smad3、p-P38MAPK and Collagen Ⅲ in the lung tissues of various groups of mice.Results Compared with the Con group,the lung tissue of DM group showed significant fibrosis changes,and the expression of TGF-1,p-Smad3,p-P38MAPK and Collagen Ⅲ proteins in the lung tissue was significantly increased(P＜0.01).After Cur treatment,the pulmonary fibrosis of DMmice was significantly improved,and the protein expression of TGF-1,p-Smad3,p-P38MAPKand CollagenⅢin the lung tissue was decreased(P＜0.05).Conclusion Cur can significantly improve pulmonary fibrosis in STZ-induced DMmice by reducing the expression of fibrosis-related protein CollagenⅢ,which may be related to the inhibition of TGF-b1/Smad/P38MAPK signaling pathway.
关键词
姜黄素/糖尿病肺纤维化/转化生长因子-1/纤维化Key words
Curcumin/Diabetes pulmonary fibrosis/TGF-1/Fibrosis引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
万方数据