摘要
目的 评估金银花对鸽新城疫病毒(NDV)感染肉鸽器官组织、血清免疫指标及抗氧化能力的影响,以期为金银花作为鸽新城疫的潜在预防和治疗药物提供科学依据.方法 将80 只35 日龄的健康鸽(未免疫NDV疫苗)随机分为2 个试验组和2 个对照组,即金银花预防组(P)、金银花治疗组(H)、攻毒对照组(V)和空白对照组(N).P组和H组分别于35 日龄、42 日龄添加1.2%金银花提取物饮水7d,P组、H组和V组同时于 42 日龄肌内注射鸽NDV4416 毒株(0.5 mL/只).于攻毒后第7 天每组随机剖杀3 只鸽子,观察并记录脑、肝、脾、肺的剖检病变和组织病变.于攻毒后第3、7 天每组随机采集3 只鸽子的血液,用ELISA和生化法测定血清免疫指标(IgA、IgG、C3、IFN-γ)和抗氧化能力指标(SOD、T-AOC)含量.结果 相较于V组,P组和H组鸽子脑、肝、脾、肺的剖检病变和组织病明显减少,血清特异性免疫指标(IgA、IgG)分泌水平大体升高,非特异性免疫指标(C3、IFN-γ)和抗氧化能力指标(SOD、T-AOC)分泌水平均显著上升(p＜0.05).结论 使用1.2%金银花提取物作为抗病毒药物可有效缓解鸽NDV对肉鸽器官组织造成的损伤,增强机体免疫力,提高机体抗氧化能力,其中金银花预防组的效果优于治疗组.
Abstract
Objective To evaluate the effects of Flos Lonicerae on organ tissue,serum immune indexes,and antioxidant capacity of pigeons infected with Newcastle diseuse virus(NDV)to provide a scientific basis for Flos Lonicerae as a potential preventive and therapeutic drug for Newcastle disease in pigeons.Method 80 35-day-old healthy pigeons(not immunized with NDV vac-cine)were randomly divided into two experimental groups and two control groups,namely honeysuckle prevention group(P),hon-eysuckle treatment group(H),challenge control group(V)and blank control group(N).Groups P and H were fed water for seven days supplemented with 1.2%honeysuckle extract at 35 days of age and 42 days of age,respectively.Groups P,H,and V were simultaneously injected with pigeon NDV4416 strain(0.5 mL/bird)at 42 days of age.On the seventh day after the challenge,three pigeons were randomly killed in each group.The pathological changes of brain,liver,spleen,and lung were observed and re-corded.On the third and seventh day after the challenge,the blood of 3 pigeons in each group was collected randomly,and the se-rum immune indexes(IgA,IgG,C3,IFN-γ)and antioxidant indexes(SOD,T-AOC)were determined by ELISA and biochemical methods.Result Compared with group V,the pathological changes and tissue diseases of the brain,liver,spleen,and lung of pi-geons in groups P and H were significantly reduced,the secretion levels of serum-specific immune indexes(IgA and IgG)were generally increased,and the secretion levels of non-specific immune indexes(C3,IFN-γ)and antioxidant capacity indexes(SOD,T-AOC)were significantly increased(p＜0.05).Conclusion The use of 1.2%honeysuckle extract as the antiviral drug can ef-fectively alleviate the damage of pigeon NDV on the organ tissue,enhance the immune function,and improve the antioxidant ca-pacity of the body.The effect of the prevention group was better than that of the treatment group.
基金项目
2021年广西农业职业技术学院院级课题(YKJ2117)
2023年广西高校中青年教师科研基础能力提升项目(2023KY1237)
维普
万方数据