Effects of Honeysuckle Flower on organ tissue,serum immune indexes,and antioxidant capacity of pigeon infected with NDV
Objective To evaluate the effects of Flos Lonicerae on organ tissue,serum immune indexes,and antioxidant capacity of pigeons infected with Newcastle diseuse virus(NDV)to provide a scientific basis for Flos Lonicerae as a potential preventive and therapeutic drug for Newcastle disease in pigeons.Method 80 35-day-old healthy pigeons(not immunized with NDV vac-cine)were randomly divided into two experimental groups and two control groups,namely honeysuckle prevention group(P),hon-eysuckle treatment group(H),challenge control group(V)and blank control group(N).Groups P and H were fed water for seven days supplemented with 1.2%honeysuckle extract at 35 days of age and 42 days of age,respectively.Groups P,H,and V were simultaneously injected with pigeon NDV4416 strain(0.5 mL/bird)at 42 days of age.On the seventh day after the challenge,three pigeons were randomly killed in each group.The pathological changes of brain,liver,spleen,and lung were observed and re-corded.On the third and seventh day after the challenge,the blood of 3 pigeons in each group was collected randomly,and the se-rum immune indexes(IgA,IgG,C3,IFN-γ)and antioxidant indexes(SOD,T-AOC)were determined by ELISA and biochemical methods.Result Compared with group V,the pathological changes and tissue diseases of the brain,liver,spleen,and lung of pi-geons in groups P and H were significantly reduced,the secretion levels of serum-specific immune indexes(IgA and IgG)were generally increased,and the secretion levels of non-specific immune indexes(C3,IFN-γ)and antioxidant capacity indexes(SOD,T-AOC)were significantly increased(p<0.05).Conclusion The use of 1.2%honeysuckle extract as the antiviral drug can ef-fectively alleviate the damage of pigeon NDV on the organ tissue,enhance the immune function,and improve the antioxidant ca-pacity of the body.The effect of the prevention group was better than that of the treatment group.