Objective To evaluate the effects of Flos Lonicerae on organ tissue,serum immune indexes,and antioxidant capacity of pigeons infected with Newcastle diseuse virus(NDV)to provide a scientific basis for Flos Lonicerae as a potential preventive and therapeutic drug for Newcastle disease in pigeons.Method 80 35-day-old healthy pigeons(not immunized with NDV vac-cine)were randomly divided into two experimental groups and two control groups,namely honeysuckle prevention group(P),hon-eysuckle treatment group(H),challenge control group(V)and blank control group(N).Groups P and H were fed water for seven days supplemented with 1.2%honeysuckle extract at 35 days of age and 42 days of age,respectively.Groups P,H,and V were simultaneously injected with pigeon NDV4416 strain(0.5 mL/bird)at 42 days of age.On the seventh day after the challenge,three pigeons were randomly killed in each group.The pathological changes of brain,liver,spleen,and lung were observed and re-corded.On the third and seventh day after the challenge,the blood of 3 pigeons in each group was collected randomly,and the se-rum immune indexes(IgA,IgG,C3,IFN-γ)and antioxidant indexes(SOD,T-AOC)were determined by ELISA and biochemical methods.Result Compared with group V,the pathological changes and tissue diseases of the brain,liver,spleen,and lung of pi-geons in groups P and H were significantly reduced,the secretion levels of serum-specific immune indexes(IgA and IgG)were generally increased,and the secretion levels of non-specific immune indexes(C3,IFN-γ)and antioxidant capacity indexes(SOD,T-AOC)were significantly increased(p＜0.05).Conclusion The use of 1.2%honeysuckle extract as the antiviral drug can ef-fectively alleviate the damage of pigeon NDV on the organ tissue,enhance the immune function,and improve the antioxidant ca-pacity of the body.The effect of the prevention group was better than that of the treatment group.
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