首页|金银花对鸽新城疫病毒感染肉鸽器官组织、血清免疫指标及抗氧化能力的影响

金银花对鸽新城疫病毒感染肉鸽器官组织、血清免疫指标及抗氧化能力的影响

扫码查看
目的 评估金银花对鸽新城疫病毒(NDV)感染肉鸽器官组织、血清免疫指标及抗氧化能力的影响,以期为金银花作为鸽新城疫的潜在预防和治疗药物提供科学依据.方法 将80 只35 日龄的健康鸽(未免疫NDV疫苗)随机分为2 个试验组和2 个对照组,即金银花预防组(P)、金银花治疗组(H)、攻毒对照组(V)和空白对照组(N).P组和H组分别于35 日龄、42 日龄添加1.2%金银花提取物饮水7d,P组、H组和V组同时于 42 日龄肌内注射鸽NDV4416 毒株(0.5 mL/只).于攻毒后第7 天每组随机剖杀3 只鸽子,观察并记录脑、肝、脾、肺的剖检病变和组织病变.于攻毒后第3、7 天每组随机采集3 只鸽子的血液,用ELISA和生化法测定血清免疫指标(IgA、IgG、C3、IFN-γ)和抗氧化能力指标(SOD、T-AOC)含量.结果 相较于V组,P组和H组鸽子脑、肝、脾、肺的剖检病变和组织病明显减少,血清特异性免疫指标(IgA、IgG)分泌水平大体升高,非特异性免疫指标(C3、IFN-γ)和抗氧化能力指标(SOD、T-AOC)分泌水平均显著上升(p<0.05).结论 使用1.2%金银花提取物作为抗病毒药物可有效缓解鸽NDV对肉鸽器官组织造成的损伤,增强机体免疫力,提高机体抗氧化能力,其中金银花预防组的效果优于治疗组.
Effects of Honeysuckle Flower on organ tissue,serum immune indexes,and antioxidant capacity of pigeon infected with NDV
Objective To evaluate the effects of Flos Lonicerae on organ tissue,serum immune indexes,and antioxidant capacity of pigeons infected with Newcastle diseuse virus(NDV)to provide a scientific basis for Flos Lonicerae as a potential preventive and therapeutic drug for Newcastle disease in pigeons.Method 80 35-day-old healthy pigeons(not immunized with NDV vac-cine)were randomly divided into two experimental groups and two control groups,namely honeysuckle prevention group(P),hon-eysuckle treatment group(H),challenge control group(V)and blank control group(N).Groups P and H were fed water for seven days supplemented with 1.2%honeysuckle extract at 35 days of age and 42 days of age,respectively.Groups P,H,and V were simultaneously injected with pigeon NDV4416 strain(0.5 mL/bird)at 42 days of age.On the seventh day after the challenge,three pigeons were randomly killed in each group.The pathological changes of brain,liver,spleen,and lung were observed and re-corded.On the third and seventh day after the challenge,the blood of 3 pigeons in each group was collected randomly,and the se-rum immune indexes(IgA,IgG,C3,IFN-γ)and antioxidant indexes(SOD,T-AOC)were determined by ELISA and biochemical methods.Result Compared with group V,the pathological changes and tissue diseases of the brain,liver,spleen,and lung of pi-geons in groups P and H were significantly reduced,the secretion levels of serum-specific immune indexes(IgA and IgG)were generally increased,and the secretion levels of non-specific immune indexes(C3,IFN-γ)and antioxidant capacity indexes(SOD,T-AOC)were significantly increased(p<0.05).Conclusion The use of 1.2%honeysuckle extract as the antiviral drug can ef-fectively alleviate the damage of pigeon NDV on the organ tissue,enhance the immune function,and improve the antioxidant ca-pacity of the body.The effect of the prevention group was better than that of the treatment group.

honeysucklepigeonNewcastle dissease virusorgan tissueserum immune indexantioxidant capacity

莫红芳、石宗承、何东贤、覃振斌、虞霖田、吕鑫、熊晓妍

展开 >

广西农业职业技术大学,广西 南宁 530007

广西大学,广西 南宁 530004

广西农垦良丰农场有限公司,广西 桂林 541006

中国农业科学院哈尔滨兽医研究所,黑龙江 哈尔滨 150069

展开 >

金银花 新城疫病毒 器官组织 血清免疫指标 抗氧化能力

2021年广西农业职业技术学院院级课题2023年广西高校中青年教师科研基础能力提升项目

YKJ21172023KY1237

2024

畜禽业
四川省科学技术信息研究所

畜禽业

影响因子:0.15
ISSN:1008-0414
年,卷(期):2024.35(1)
  • 15