Cloning and sequencing of the gene encoding chicken interleukin-15
Objective To express the chicken interleukin-15(ChIL-15)gene in a prokaryotic expression system by means of genetic engineering to provide a scientific basis for the application of ChIL-15.Methods Primers were designed based on the CHER-15 gene in the GenBank of the National Center for Biological Information(NCBI),and PCR was utilized to amplify the target gene fragment.Result The ChIL-15 gene containing the coding region was successfully amplified from the spleen of chickens,with a length of 581 bp.Sequencing and comparison showed that the ChIL-15 gene was successfully cloned.The extrac-ted plasmids were identified by enzyme digestion,PCR,sequencing,and amino acid coding and were consistent with the registra-tion number AF139097.1 in GenBank.The nucleotide homology was 99.82%.Conclusion The ChIL-15 gene fragment suc-cessfully amplified by the PCR method has high homology and is consistent with the published gene sequence.This provides a scientific basis for further research on the function and application of the ChIL-15 gene.
NETL
NSTL
万方数据
维普
