牛环曲病毒云南流行株N基因序列分析
Detection of bovine torovirus in some areas of Yunnan Province and analysis of its N gene sequence
吴绍伟 1申爱华 2余世祥 1陶灿松 1李维超 1高亮 3张以芳4
作者信息
- 1. 德宏州尚善品味农业有限公司,云南 梁河 679200
- 2. 德宏州梁河县畜牧站,云南 梁河 679200
- 3. 云南农业大学动物医学院,云南 昆明 650201
- 4. 德宏州尚善品味农业有限公司,云南 梁河 679200;云南农业大学动物医学院,云南 昆明 650201
- 折叠
摘要
为深入了解云南省牛环曲病毒(bovine torovirus,BToV)的流行现状及遗传进化趋势,2021 年3 月—2023 年6 月采集云南省9 市 15 个养牛场不同年龄段的655 份牛粪便样品,进行BToV RT-PCR检测,并对其中具有代表性的 6 份样品进行N基因测序分析.结果显示:BToV阳性检出率为 10.84%(71/655);扩增得到6 条完整的N基因序列,其同源性在98.4%～100%,与国内外参考毒株相比发现,6 个毒株 N基因与我国四川、黑龙江毒株同源性高,亲缘关系较近,可能是由引种、调运或动物产品交易传播而来;发现2 个毒株发生氨基酸突变,可能是由于云南独特的气候和不同的饲养条件造成的.结果表明:牛环曲病毒在云南存在范围较广,须密切注意监测、加强检疫,同时加快疫苗研发.
Abstract
In order to gain a deeper understanding of the prevalence and genetic evolution trends of bovine circovirus(BToV)in Yunnan Province,655 cow feces samples from 15 cattle farms in 9 cities of Yunnan Province were collected from March 2021 to February 2023 and BToV RT-PCR detection was performed.Six representative samples were analyzed for Ngene sequencing.The results showed that the positive detection rate of BToV was 10.84%(71/655);Six complete Ngene sequences were amplified,with homology ranging from98.4%to100%.Compared with domestic and foreign reference strains,it was found that the Ngenes of the six strains had high homology and close genetic relationships with domestic Sichuan and Heilongjiang strains,possibly origi-nating from their transmission;two strains of the virus were found to have amino acid mutations,possibly due to the unique climate and different feeding conditions in Yunnan.The results indicate that bovine circovirus has been widely spread in Yunnan,possibly through introduction,transportation,or animal product trading.So,it is necessary to strengthen quarantine,pay attention to monito-ring,and accelerate vaccine development.
关键词
牛环曲病毒/流行病学调查/遗传进化趋势/N基因Key words
bovine torovirus/epidemiological investigation/genetic evolution trend/Ngene引用本文复制引用
出版年
2024
NETL
NSTL
万方数据