Establishment and application of multiplex real-time PCR method for rapid detection of three animal-derived components in powdered posterior pituitary
Objective To determine the porcine,bovine and sheep derived components in powdered posterior pituitary by a mul-tiplex real-time polymerase chain reaction(PCR)method.Methods The DNA was extracted by CTAB method.The mitochon-dria genes were used as target genes to design specific primers and Taq Man probes.The developed method was validated and ap-plied to detect whether the animal-derived components in the samples were consistent with those indicated.Results The mini-mum detection concentration was 10 pg/μL of template DNA.The3 batches of powdered posterior pituitary samples were identified by the successfully established method,and only pig source components were detected.Conclusion The multiplex real-time PCR method can be used for the detection of porcine,bovine and sheep derived components in powdered posterior pituitary.
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