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花生红衣对小鼠抗氧化能力研究

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目的 通过研究花生红衣对小鼠增重和抗氧化效果,为花生红衣在畜禽养殖中深入开发应用提供数据支持.方法 在小鼠日粮中分别添加3%生花生红衣(试验Ⅰ组)、5%生花生红衣(试验Ⅱ组)和3%熟花生红衣(试验Ⅲ组)进行饲喂试验,并测定小鼠体质量、平均日增质量和血清与肝脏组织中超氧化物气化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量.结果 花生红衣对小鼠体质量和平均日增质量无显著影响(p>0.05);对小鼠血清和肝脏组织中T-SOD和GSH-Px活性与MDA含量具有显著影响.对于T-SOD活性而言,试验Ⅰ组和试验Ⅱ组小鼠血清中T-SOD活性显著高于对照组和试验Ⅲ组(p<0.05),而试验Ⅲ组小鼠血清T-SOD活性与对照组相比提高2.1%,未达显著水平(p>0.05);试验Ⅱ组肝脏组织中T-SOD活性显著高于试验Ⅰ组、试验Ⅲ组和对照组(p<0.05),试验Ⅰ组、试验Ⅲ组肝脏组织中T-SOD活性与对照组相比略有提升,但未达显著水平(p>0.05).对于GSH-Px活性而言,试验Ⅰ组和试验Ⅱ组小鼠血清中GSH-Px活性显著高于对照组和试验Ⅲ组(p<0.05),而试验Ⅲ组小鼠血清GSH-Px活性与对照组相比提高 6.89%,未达显著水平(p>0.05);试验Ⅱ组小鼠肝脏组织中GSH-Px活性显著高于对照组和试验Ⅰ组(p<0.05),但与试验Ⅲ组无显著差异(p>0.05),试验Ⅲ组小鼠肝脏组织中GSH-Px活性与对照组相比显著提高(p<0.05).对于MDA含量而言,3 个试验组小鼠血清和肝脏组织中MDA含量均显著低于对照组小鼠血清中MDA含量(p<0.05).结论 花生红衣对小鼠具有较好的抗氧化效果,生花生红衣的抗氧化效果好于熟花生红衣;5%生花生红衣(试验Ⅱ组)抗氧化效果最佳.故在畜禽养殖中添加5%的生花生红衣为宜.
Study on antioxidant capacity of peanut red coating in mice
The experiment aimed to provide data support for the further development and application of peanut red coating in livestock and poultry breeding by studying the weight gain and antioxidant effects of peanut red coating on mice.The feeding ex-periment was conducted by adding 3%raw peanut red coating(trial group Ⅰ),5%raw peanut red coating(trial group Ⅱ)and 3%cooked peanut red coating(trial group Ⅲ)to the mice diet,and determining the body weight,daily weight gain,and the activities of superoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)content in serum and liver tis-sues of mice.The results showed that peanut red coating had no significant effects on body weight and daily weight gain(p>0.05).The activity of T-SOD and GSH-Px and the content of MDA in serum and liver of mice were significantly affected.As for T-SOD activity,the T-SOD activity in serum of mice in trial groups Ⅰ and Ⅱ was significantly higher than that in control group and trial group Ⅲ(p<0.05),while the T-SOD activity in serum of mice in trial group Ⅲ was increased by 2.1%compared with the control group,which was not significant(p>0.05).The T-SOD activity in liver tissues of trial group Ⅱ was significantly higher than that of trial groups Ⅰ,Ⅲ and the control group(p<0.05),and the T-SOD activity in liver tissues of trial groups Ⅰ and Ⅲ was slightly increased compared with the control group,but did not reach a significant level(p>0.05).As for the GSH-Px activity,the serum GSH-Px activity in trial groups Ⅰ and Ⅱ was significantly higher than that in the control group and trial group Ⅲ(p<0.05),while the serum GSH-Px activity in trial group Ⅲ was increased by 6.89%compared with the control group(p>0.05).The activity of GSH-Px in liver tissues of trial group Ⅱ was significantly higher than that of the control group and tri-al group Ⅰ(p<0.05),but there was no significant difference compared with trial group Ⅲ(p>0.05),and the activity of GSH-Px in liver tissues of trial group Ⅲ was significantly higher than that of control group(p<0.05).In terms of MDA content,the MDA content in serum and liver tissue of mice in three trial groups was significantly lower than that in the control group(p<0.05).This showed that peanut red coating had better antioxidant effect on mice,and the antioxidant effect of raw peanut red coating was better than that of cooked peanut red coating.The 5%raw peanut red(trial group Ⅱ)had the best antioxidant effect.It is advisable to add 5%of raw peanut red coating in livestock and poultry farming.

peanut red coatingmouseserumliver tissueantioxidant capacity

李成会、赵娜、朱莲英

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唐山师范学院生命科学系/唐山市动物营养与饲料工程重点实验室,河北 唐山 063000

花生红衣 小鼠 血清 肝脏组织 抗氧化能力

唐山师范学院科学研究基金项目唐山师范学院一流学科建设项目

2021B382022XXK01

2024

畜禽业
四川省科学技术信息研究所

畜禽业

影响因子:0.15
ISSN:1008-0414
年,卷(期):2024.35(8)