Mutation breeding of β-glucanase high production strain
Using Trichoderma sp.As original strain,a mutant strain UN123 with high yield β-glucanase was obtained by UN and photoreactivation.The β-glucanase activity of Trichoderma sp.UN123 in shake flask was above 10 000 U/Ml,its optimum tem-perature was 40℃.The β-glucanase activity of Trichoderma sp.UN123 maintained above 80%even given heat preservation of 2 h under the temperature of 90℃.Its optimum pH was 3.0.The β-glucanase activity of Trichoderma sp.UN123 maintained above 80%under the condition of pH 2.0～7.0 of 2 h and showed god tolerance to pepsin,trypsin,and chymotrypsin.
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