The effect of adding coated sodium butyrate in feed on the prokaryotic microbiota of cecum of chicks
Objective To investigate the impact of adding coated sodium butyrate to the feed on the diversity of prokaryotic mi-crobiota of cecum of chicks.Method This study selected one-day-old Roman Brown chicks randomly divided into a control group and an experimental group.The control group received a standard diet,while the experimental group's feed contained 1 500 mg/kg of 30%coated sodium butyrate,with all other rearing conditions being the same,and recorded the chick growth data of the two groups in 12 days,18 days,24 days and 30 days respectively.After one month,randomly selected healthy growing chicks were euthanized,and their cecal contents were immediately collected.Microbial total DNA was extracted,and the V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified using PCR and subjected to high-throughput sequencing on the Illumina HiSeq platform.Result The analysis results showed that the chicks in the experimental group,with the addition of coa-ted sodium butyrate,exhibited a significant increase in body weight during the later stages of growth.α diversity analysis indicated that there were no significant differences in ACE,Chao,Simpson,and Shannon indices between the two groups(p>0.05).At the phylum level,Bacteroidetes,Firmicutes,and Proteobacteria were the dominant phyla in the cecal bacterial community,with the ex-perimental group having a higher proportion of Bacteroidetes and a lower proportion of Firmicutes.At the genus level,the experi-mental group showed a significant increase in the abundance of Bacteroides and Lactobacillus(p<0.05),while the abundance of Desulfovibrio and Anaerobiospirillum decreased significantly(p<0.05).Conclusion As a novel non-antibiotic growth-promoting feed additive,coated sodium butyrate did not significantly affect the diversity of prokaryotic microbiota of cecum of chicks.Howev-er,it did have a significant impact on the proportions of microbial genera,altering the composition of the cecum microbe.This may contribute to improving gut health and cecal microbial communities.
万方数据
维普
