Degradation and Dynamic Distribution of MDMB-4en-PINACA and Its Metabolites in Rats
To establish a high performance liquid chromatography-triple quadrupole tandem mass spectrometry(HPLC-MS/MS)method for the detection of MDMB-4en-PINACA and its metabolites in biological samples,we studied the degradation and dynamic distribution of MDMB-4en-PINACA,its hydrolytic metabolites(M1),and its dealkylated metabolites(M2)in rats.Five SD rats were divided into five groups,one group was the blank control group,and the other four groups were placed in clean metabolic cages after intragastric administration of MDMB-4en-PINACA.Urine samples were collected from each group for duration of 1 to 15 days respectively.Sixty SD rats were randomly divided into 15 groups;one group was used as the blank control group,and the other 14 groups were administrated with MDMB-4en-PINACA by gavage.They were killed at different time points(15 min,30 min,45 min,1 h,1.5 h,2 h,3 h,4 h,5 h,6 h,7 h,8 h,10 h,and 12 h).Blood,urine,and visceral organs(heart,liver,spleen,lungs,kidneys,brain,and muscles)were collected immediately.The mass concentrations of MDMB-4en-PINACA and metabolites Ml and M2 in the tissues,blood,and urine were detected by HPLC-MS/MS.Following the administration of MDMB-4en-PINACA into the rat's body,it was rapidly distributed and metabolized.Highest concentration was reached in all the tissues and blood samples within 15 min.The distribution characteristics of MDMB-4en-PINACA in the body were as follows:brain＞spleen＞blood＞lung＞heart＞liver＞kidney＞muscle＞urine.The distribution characteristics of Ml were as follows:blood＞spleen＞lung＞brain＞liver＞kidney＞heart＞muscle＞urine.The distribution of M2 was as follows:blood＞liver＞heart＞lung＞brain＞spleen＞kidney＞muscle＞urine.Within 12 hours,MDMB-4en-PINACA and its metabolites were distributed and degraded in different tissues.The contents of MDMB-4en-PINACA and its metabolites were less in the urine monitoring for 1 to 15 days.The established method has the advantages of simple operation,low detection limit,high recovery,and good reproducibility.This method is suitable for the detection of MDMB-4en-PINACA and its metabolites in biological samples.Following the intragastric administration of MDMB-4en-PINACA to rats,its distribution,degradation,and metabolites were monitored to provide a scientific basis for the MDMB-4en-PINACA test in vivo.
forensic toxicologyMDMB-4en-PINACAhigh performance liquid chromatography-triple quadrupole tandem mass spectrometry(HPLC-MS/MS)degradation ruledynamic distribution
万方数据
维普
