Application of Anti-contamination Amplification Reagents in Forensic Biological Evidence Examination
Objective and accurate forensic genetic analysis results are the basis for making accurate identification opinions.With the increasing sensitivity of testing equipment and amplification kits,the pressure to prevent laboratory pollution is increasing.Among them,the contamination of PCR amplification products is the most difficult type of pollution to prevent.This research introduces an anti-pollution amplification kit,which contains 16 short tandem repeats(STR)locus,one sex identification locus(Amel)and one Y chromosome insertion deletion locus(Indel).Anti-pollution amplification kit can obtain DNA fragment containing uracil base through STR multiplex amplification,and this type of DNA fragment can be efficiently hydrolyzed by uracil DNA glycosylase(UDG enzyme)at 50℃.An additional 50℃ incubation before each new round of PCR amplification can completely eliminate the pollution threat of previous amplification products to the results.After testing,the anti-pollution amplification kit has excellent anti-pollution ability,and uracil base does not change the DNA typing results.Its detection sensitivity does not decrease;the stability of DNA product fragments does not decrease;and subsequent electrophoresis analysis is not affected.This reagent kit can effectively eliminate contamination of amplification products.
forensic biologydeoxyribosideuracil DNA glycosylasecontamination
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