毛发中甲基苯丙胺检验的样品前处理方法研究
Study on Sample Pretreatment Methods for Determination of Methamphetamine in Hair Samples
于交远 1包少魁 2王爱华 3董林沛 3王瑞花 3邹波 3李佳宜 3吴小军3
作者信息
- 1. 公安部鉴定中心,北京 100038;呼伦贝尔市公安局刑事侦查支队,内蒙古呼伦贝尔 021000
- 2. 呼伦贝尔市公安局刑事侦查支队,内蒙古呼伦贝尔 021000
- 3. 公安部鉴定中心,北京 100038
- 折叠
摘要
针对毛发中甲基苯丙胺呈阳性的样本,本文分别采用不同的清洗、研磨、提取方法,制备样品提取液进样分析,探索出应用液相色谱-串联质谱法(LC-MS/MS)分析毛发中甲基苯丙胺的最优前处理条件.根据保留时间、特征离子对及甲基苯丙胺丰度比进行定性,特征离子对峰面积进行定量,优化前处理方法.结果表明,用甲醇研磨提取时,毛发中甲基苯丙胺具有最大的显现浓度.本文系统研究了液相色谱-质谱联用法(LC-MS/MS)分析毛发中甲基苯丙胺前处理方法对结果的影响,探索出一种用于毛发样品中甲基苯丙胺定性、定量检验的最优前处理条件.
Abstract
By analyzing and studying the hair samples that contain methamphetamine,the paper aims to explore the optimal pretreatment conditions when determining methamphetamine in hair using liquid chromatography-tandem mass spectrometry(LC-MS/MS).Different cleaning,grinding,and extraction conditions were compared to optimize the pretreatment method.Retention time,characteristic ion pairs and their relative abundance were used for qualitative determination.The peak area of characteristic ion pairs was used for quantification.The results showed that the concentration of methamphetamine in hair was the highest when extracted by methanol grinding.In this paper,the impact of pretreatment methods on the results of methamphetamine analysis in hair by LC-MS/MS was systematically studied and analyzed,and an optimal pretreatment condition was explored for the qualitative and quantitative detection of methamphetamine in hair samples.
关键词
法医毒物学/毛发/甲基苯丙胺/样品前处理Key words
forensic toxicology/hair samples/methamphetamine/sample pretreatment引用本文复制引用
基金项目
公安部技术研究计划(2020JSYJC11)
中央级公益性科研院所基本科研业务费专项(2021JB007)
出版年
2024
国家科技期刊平台
NSTL
万方数据