DNATyper? 21与Verifiler? Plus试剂盒对常规案件检材检验的比较
Comparative Evaluation of DNATyper? 21 and Verifiler? Plus Kit in Forensic DNA Casework
王剑 1马云龙 1余政梁 2龚政 3高馨玉 4杨亮 5张丽娜6
作者信息
- 1. 黑龙江省公安厅,哈尔滨 150008
- 2. 公安部鉴定中心,北京 100038
- 3. 哈尔滨市公安局,哈尔滨 150010
- 4. 齐齐哈尔市公安局,黑龙江齐齐哈尔 161005
- 5. 牡丹江市公安局,黑龙江牡丹江 157399
- 6. 哈尔滨铁路公安局,哈尔滨 150001
- 折叠
摘要
本文通过比较DNATyper™ 21与Verifiler™ Plus两种STR检测试剂盒对各类案件检材的检验能力,探讨DNATyper™ 21试剂盒在常规案件检验中应用的可靠性.取0.031 25、0.062 5、0.125、0.25、0.5、1.0ng/μL基因组标准品对两种试剂盒进行灵敏度测试;应用DNATyper™ 21与Verifiler™ Plus试剂盒分别对1 056例常规案件检材DNA进行检验;对同一样本等位基因进行一致性比较.结果表明,两种试剂盒均可以成功检测出模板浓度在0.062 5 ng/μL以上的标准DNA.在检验的1 056例样本中,DNATyper™ 21试剂盒共检出881例,检出率为83.4％;Verifiler™ Plus试剂盒检出892例,检出率为84.5％.统计学结果表明两种试剂盒检出率无统计学差异,且同一样本相同基因座得到的等位基因分型一致.综上,DNATyper™ 21试剂盒对各类常规检材具有良好的检验能力,可应用于日常案件检验.
Abstract
The DNATyper™ 21 amplification kit is a 5-dye multiplex assay,which is widely used in construction of the Chinese National autosomal DNA database.However,the DNATyper™ 21 kit has not been extensively used in forensic DNA casework.Therefore,this study aimed to elucidate the reliability of the DNATyper™ 21 kit compared with Verifiler™ Plus kit and to assess its applicability on forensic analysis.Standard DNA samples with concentrations of 0.031 25,0.062 5,0.125,0.25,0.5 and 1.0 ng/μL were used for sensitivity testing.DNATyper™ 21 kit and Verifiler™ Plus kit were utilized to detect the DNA extracted from 1056 casework evidence samples and the allele consistency of the same sample was analyzed.The results showed that both kits could successfully detect standard DNA with a template concentration above 0.062 5 ng/μ.L.For casework evidence samples,DNATyper™ 21 kit successfully detected 881 cases out of 1 056,and the detection rate is 83.4％,while Verifiler™ Plus kit successfully detected 892 cases out of 1 056,and the detection rate is 84.5％.The statistical results showed that there was no significant difference in the detection rate between the two kits,and the alleles obtained at the same locus of the same sample were completely consistent.In conclusion,the DNATyper™ 21 is considered to have the capability to be used for STR analysis of casework evidence samples.
关键词
法医物证学/DNATyper™/21试剂盒/Verifiler™Plus试剂盒/比较研究/生物检材Key words
forensic biology/DNATyper™ 21 kit/Verifiler™ Plus kit/comparative evaluation/casework evidence samples引用本文复制引用
基金项目
法医遗传学公安部重点实验室开放基金(2020FGKFKT03)
出版年
2024
国家科技期刊平台
NSTL
万方数据