Evaluation of a Novel 32 X-STR Loci Multiplex System in the Forensic Application
The AGCU X Plus STR system is a newly developed multiplex PCR kit that detects 32 X-chromosomal STR loci simultaneously.These are DXS6807,DXS9895,linkage group 1(DXS10148,DXS10135,DXS8378),DXS9902,DXS6795,DXS6810,DXS10159,DXS10162,DXS10164,DXS7132,linkage group 2(DXS10079,DXS10074,DXS10075),DXS981,DXS6800,DXS6803,DXS6809,DXS6789,DXS7424,DXS101,DXS7133,GATA172D05,GATA165B12,linkage group 3(DXS10103,HPRTB,DXS10101),GATA31E08 and linkage group 4(DXS8377,DXS10134,DXS7423).A major advantage of this kit is that it takes into account linkage between loci,in addition to detecting more X-STR loci.In order to evaluate the forensic application of 32 X-STR fluorescence amplification system,PCR settings,sensitivity,species specificity,stability,DNA mixtures,concordance,stutter,sizing precision,and population genetics investigation were evaluated according to the Scientific Working Group on DNA Analysis Methods(SWGDAM)developmental validation guidelines.The study showed that the genotyping results of each locus were significantly accurate when the DNA template was at least 62.5 pg.Complete profiles were obtained for the 1∶1 and 1∶3 combinations.A total of 209 unrelated individuals from Southern Chinese Han community,consisting of 84 females and 125 males,were selected for population studies,and 285 allele profiles were detected from 32 X-STR loci.The polymorphism information content(PIC)ranged from 0.272 1 in DXS6800,to 0.910 5 in DXS10135,with an average of 0.679 8.DXS10135(PIC=0.910 5)was the most polymorphic locus,with discrimination power(DP)of 0.916 4 and 0.987 1 for the male and female.The cumulative PDF,PDM,MECtrio and MECduo values were all greater than 0.999 999 999.There were 78 different DXS10103-HPRTB-DXS10101 haplotypes among the 125 males,and the haplotype diversity was 0.9810.There was no significant difference in the cumulative PDF,PDM,MECtrio and MECduo values whether considering linkage or not.In summary,the new X-STR multiplex typing system is effective and reliable,which can be useful in human genetic analysis and kinship testing as a potent complement to autosomal STR typing.
国家科技期刊平台
NSTL
万方数据
