Detection of Multiple Y-STRs Allele Dropout and AZF Analysis
In this paper,the characteristics of allele typing deletion of Y-STR loci and its correlation with AZF(azoospermia factor)deletion were discussed,which could provide reference for forensic practice.Y-STR kits(Yfiler Platinum,SureID PathFinder Plus)were used to analyze the blood samples of 23 461 male family members.A total of 14 cases with 4 or more Y-STR allele-dropout samples were found.Meanwhile,Sequence-tagged site(STS)was detected with Y chromosome microdeletion detection kit,and the deletion of AZF region of Y chromosome was evaluated according to the deletion STS.The results showed that the proportion of multiple Y-STR typing missing was 0.059 7%(14/23461),with 1 case of short arm and 13 cases of long arm,which were from different families with different types.STS deletion was detected in the AZF region in 13 long arm multiple typing deletion samples,and no abnormality was detected in 1 short arm multiple typing deletion sample.This study suggests that there is a correspondence between multiple STR allele dropouts in the long arm of Y chromosome and microdeletions in AZF region,and the biological basis of sterility exists in these typing individuals.
国家科技期刊平台
NSTL
万方数据
