There are many kinds of toad toxins,which have strong toxicity,high medicinal value,and great potential for application.The toxic components in toad toxins can be categorized into bufadienolides and indole alkaloids,according to their chemical compositions,which are cardiotoxic and hallucinogenic,respectively.In addition,toad toxin is the main component in Chansu,which has an obvious inhibitory effect on several types of tumor cells.The extraction and detection methods of toad toxins are also different in different drug substrates.This study summarizes the common extraction and detection methods,hoping to provide a reference for the development of standard methods,forensic scientific toxicological testing,and formulation of related standards.