Identification of Half Sibship by Combined Application of Multiple Genetic Markers:A Case Report
At present,in genetic identification,the detection of autosomal STR loci and the calculation of half sibling index using the ITO method can infer whether there is a genetic relationship among the identified individuals.However,the ITO method sometimes cannot provide a clear judgment basis for calculating likelihood rates.Therefore,multiple genetic markers can be detected to assist in judgment and different calculation methods can be used for comprehensive analysis to obtain evidence support for determining genetic relationships.Half sibling identification is a difficult point in genetic relationship identification,especially in heterosexual half sibling relationships,which cannot be assisted by the genetic rules of X-STR or Y-STR,which further increases the risk and difficulty of identification.In the case where only two individuals participate in identification,it is also impossible to analyze through family reconstruction methods.Therefore,in a case of half sibling relationship identification,this article detected genetic markers such as autosomal STR,mtDNA,and SNP,and comprehensively used various analysis methods such as ITO,discriminant function,mitochondrial high variability region polymorphism,and state consistency based on allele frequency estimation to infer the degree of kinship.Through mutual verification of multiple genetic marker detection results,reliable identification results were finally obtained.
length of common ancestor fragmentidentification of half-sibling relationshippedigree inferencenext generation sequencing(NGS)
万方数据
维普
