The effects of flat piston uniform scavenging chamber,flat piston non-uniform scavenging chamber and pit piston non-uniform scavenging chamber on the in-cylinder flow were analyzed.Uniflow scavenging zero-dimensional model was used to qualitative analysis in-cylinder flow organizational process and influence rule for opposed-piston two-stroke engine.Results show that uniform scavenging chamber can organize swirl and increase scavenging efficiency and non-uniform scavenging chamber can organize tumble and increase turbulent kinetic energy.Scheme 3 is favorable to organize tumble and maintain the in-cylinder turbulence intensity,which is increased by 150 percent.